Bacterial biofilm influence on medical treatment of acute exacerbation of chronic rhinosinusitis

• Grant number: 11/11764-6
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2012
• End date: July 31, 2014
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Wilma Terezinha Anselmo Lima
• Grantee: Wilma Terezinha Anselmo Lima
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Fabiana Cardoso Pereira Valera ; Roberto Martinez ; Wilma Terezinha Anselmo Lima

Abstract

To date, no study has demonstrated whether the presence of biofilm-forming bacteria interfere with the evolution of patients with acute exacerbation of chronic rhinosinusitis. Still, no comparison of values of minimum inhibitory concentration (MIC) of planktonic form and inhibitory concentration of biofilms (BIC) obtained from patients with acute exacerbation of chronic rhinosinusitis has been described so far. Objectives: To assess the prevalence of biofilm-forming bacteria, compare MIC and BIC, and to investigate whether the clinical course of antibiotic therapy is influenced by the capacity of biofilm formation in patients with acute exacerbation of chronic rhinosinusitis. Methods: Bacterial identification, determination of MIC and BIC will be held from the middle meatus secretions collected from patients with acute exacerbation of chronic rhinosinusitis. MIC will be determined for amoxicillin, AMX/Clavulanate, clarithromycin and levofloxacin. The formation of biofilms as well as determination of CIB will be performed by the Calgary biofilm assay. Daily symptom scores and nasal endoscopy evaluation will be analyzed weekly to monitor the evolution of such patients and correlated with the presence of biofilm-forming bacteria, MIC and BIC. Expected results: We expected that BIC values may be significantly higher than MIC for the planktonic counterparts. Also, biofilm-related acute exacerbations in chronic rhinosinusitis may be related with worse clinical outcome during antibiotic treatment. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SABINO, HENRIQUE AUGUSTO CANTAREIRA; VALERA, FABIANA CARDOSO PEREIRA; SANTOS, DENISE VIEIRA; FANTUCCI, MARINA ZILIO; TITONELI, CAROLINA CARNEIRO; MARTINEZ, ROBERTO; ANSELMO-LIMA, WILMA T.; TAMASHIRO, EDWIN. iofilm and Planktonic Antibiotic Resistance in Patients With Acute Exacerbation of Chronic Rhinosinusiti. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, JAN 17 2022. (11/11764-6, 13/04148-2)