| Grant number: | 11/12938-8 |
| Support Opportunities: | Regular Research Grants |
| Start date: | February 01, 2012 |
| End date: | January 31, 2014 |
| Field of knowledge: | Health Sciences - Dentistry - Dental Materials |
| Principal Investigator: | Carlos Alberto de Souza Costa |
| Grantee: | Carlos Alberto de Souza Costa |
| Host Institution: | Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil |
| City of the host institution: | Araraquara |
| Associated researchers: | Josimeri Hebling Costa ; Pedro Paulo Chaves de Souza |
Abstract
The in-office bleaching is a commonly used technique because the clinical results are quickly obtained. However, in the way that this technique has been applied nowadays, the risks of irreversible deleterious effects on pulp tissue are high. Thereafter, the aim of this study is evaluated the effectiveness, cytotoxicity and molecular effects of new in-office bleaching protocols. Enamel/dentin discs, obtained from bovine incisors, will be selected by reflectance spectrofotometer, to standardize the enamel colour. Then, the discs will be submitted to 3 bleach sections, composed by 1 or 3 applications of a bleaching gel with 15 or 35% of hydrogen peroxide (HP) for 5 or 15 minutes, or with a 37% carbamide peroxide (CP) bleaching gel for 10 or 20 minutes. The colour will be analysed after each section. In order to evaluate the trans-enamel and trans-dentinal cytotoxicity, enamel/dentin discs will be adapted to artificial pulp chambers. Odontoblast like-cells MDPC-23 and primary culture of human pulp cells (HPC) will be seeded on the dentin side of the discs and the bleaching protocols will be done on enamel side. The cell metabolism, phosphatase alcalin activity and cell morphology will be analysed, and the total of HP capable to make the trans-enamel and trans-dentinal diffusion will be quantified. The genes of inflammatory mediators and differentiation markers for odontoblasts will be analysed for PCR real time, and the calcified nodule formation will be measured on HPC. The data obtained will be submitted to specific statistical tests. (AU)
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