Performance of serological tests, semen plasma agglutination, microbiological culture and PCR of semen, andrological examination for the diagnosis of brucellosis in bulls

• Grant number: 11/20263-0
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2012
• End date: January 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Jane Megid
• Grantee: Jane Megid
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Bovine brucellosis is caused by Brucella abortus recognized as chronic disease, with zoonotic potential, related to severe losses in herds, due to abortion, birth of weak calves, premature reposition of cow, reduction of meat and milk production, as well as problems in international trade of animals and products. The brucellosis in bulls is restricted to genital tract, characterized by seminal vesiculitis and inflammation to accessory glands. In contrast to cows, B. abortus infections in bulls determine low or absence of serum levels, which difficult the serodiagnosis on basis in conventional methods. The present study aims to compare the performance of serological tests of AAT, 2-ME, FC with semen plasma bonding, the microbiological culture and PCR of semen, and to assess the andrological examination in bulls bred in the central-western state of São Paulo. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NARDI JUNIOR, G.; MEGID, J.; MATHIAS, L. A.; PAULIN, L.; VICENTE, A. F.; CORTEZ, A.; LISTONI, F. J. P.; LARA, G. H. B.; MOTTA, R. G.; CHACUR, M. G. M.; et al. Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls. BIOLOGICALS, v. 48, p. 6-9, JUL 2017. (11/20263-0)