Subcutaneous tissue response of mice to triantibiotic paste indicated as intracanal medication for teeth with pulp necrosis and incomplete root formation

• Grant number: 11/19511-0
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2012
• End date: August 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Gisele Faria
• Grantee: Gisele Faria
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Recently an alternative technique has been recommended for the treatment of teeth with incomplete root formation and pulp necrosis. A triantibiotic paste (TP) consisting of ciprofloxacin, metronidazole and minocycline is used for disinfecting root canal system followed by the induction of bleeding to fill the root canal and the coronal sealing. This technique allows root canal revascularization and root formation through the normal development of the thickness, length and morphology of the root. However there is no knowledge about the effect of TP on the connective tissue. This study will evaluate the gene expression of pro- and anti-inflammatory cytokines, hypoxia-induced factor (HIF),vascular endothelial growth factor (VEGF), metalloproteases (MMPs) and their inhibitors (TIMPs) induced by this paste. Forty mice will be distributed into four groups for each experimental period of 7 and 21 days. Each animal will receive 2 polyethylene tube implants containing the substances to be evaluated in the dorsal area: Group I - TP, Group II - calcium hydroxide paste (control), Group III - empty tube and Group IV - animals that will receive no tubes. After experimental periods of 7 and 21 days the tissue "capsule" present at the open end of the tube will be removed, followed by extraction of total RNA and preparation of complementary DNA. Detection and quantification of IL-1², TNF-±, IL-17, TGF-², VEGF, HIF, MMP-2 and -9 and TIMP-1 and -2 will be done through real-time PCR. It is our expectation to provide information about TP biological action and subsidies for the ideal intracanal medication selection to be used in permanent teeth with incomplete root formation and pulp necrosis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KALATZIS SOUSA, NATALIA GUIMARAES; DE BARROS CARDOSO, CRISTINA RIBEIRO; DA SILVA, JOEIO SATANA; KUGA, MILTON CARLOS; TANOMARU-FILHO, MARIO; FARIA, GISELE. Association of matrix metalloproteinase inducer (EMMPRIN) with the expression of matrix metalloproteinases-1, -2 and -9 during periapical lesion development. ARCHIVES OF ORAL BIOLOGY, v. 59, n. 9, p. 944-953, SEP 2014. (11/19511-0)

SWERTS PEREIRA, MARISTELA SOARES; ROSSI, MARCOS A.; CARDOSO, CRISTINA RIBEIRO; DA SILVA, JOAO SANTANA; BEZERRA DA SILVA, LEA ASSED; KUGA, MILTON CARLOS; FARIA, GISELE. Cellular and Molecular Tissue Response to Triple Antibiotic Intracanal Dressing. JOURNAL OF ENDODONTICS, v. 40, n. 4, p. 499-504, APR 2014. (11/19511-0)

FARIA, GISELE; RODRIGUES, ELISANDRA MARCIA; COAGUILA-LLERENA, HERNAN; GOMES-CORNELIO, ANA LIVIA; NETO ANGELOCO, RENAN RODRIGUES; SWERTS PEREIRA, MARISTELA SOARES; TANOMARU FILHO, MARIO. Influence of the Vehicle and Antibiotic Formulation on Cytotoxicity of Triple Antibiotic Paste. JOURNAL OF ENDODONTICS, v. 44, n. 12, p. 1812-1816, DEC 2018. (11/19511-0)