Testicular degeneration treatment in ram with vitamin and mineral supplementation or low intensity laserterapy

• Grant number: 12/00040-0
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2012
• End date: April 30, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Eneiva Carla Carvalho Celeghini
• Grantee: Eneiva Carla Carvalho Celeghini
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Maria Claudia Araripe Sucupira ; Rubens Paes de Arruda

Abstract

Testicular degeneration can lead to subfertility, infertility or even sterility in rams. The major cause of this illness is the increase of testicular temperature caused by a testicular thermoregulation. Although the treatment consists in removing the causative agent, the prognosis depends on the time of exposure to the agent and the degree of; so, agents that stimulate the renewal of the seminiferous epithelium can contribute to the reversion of that status. The supplementation whit vitamin A (vit.A), vitamin E (vit. E), selenium (Se) and zinc (Zn), helps the renewal of the seminiferous epithelium and the reduction of the reactive oxygen species concentration, therefore, can be used in the treatment of testicular; on the other hand, the use of low intensity laserterapy (LT) stimulates the cellular mitosis, it also could help in the reversion of the testicular degeneration status. This project has the purpose of advocating a treatment for testicular degeneration in rams using vitamin and mineral supplementation or laserterapy. Will be used 24 rams divided in 6 treatment groups: 1)control: not degenerated and untreated, 2) not degenerated and supplemented with vitamins A and E, selenium and zinc, 3) not degenerated and treated with low intensity laserterapy, 4) degenerated and untreated, 5) degenerated and supplemented whit vitamins A and E, selenium and zinc, 6) degenerated and treated with low intensity. During the experimental period there will be weekly analysis, including: andrologic exams, testicular e retal temperature evaluation, sonografic evaluation of the testicular parenchyma (B-mode) and testicular vascularization (Doppler mode), testosterone and cortisol mensuration. It will be preconized testicular biopisis in strategic moments. The data will be submited ta a variance analysis employing the statistical program Statistical Analysis System (SAS Institute Inc., 2004), adding the factor repeated measurements over time, the probabilites of interections with the test time will be determined by the Greenhouse-Geisse test, using the REPEATED command generated by the GLM procedure (PROC GLM do SAS). The significance level will be 5%. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES ALVES, MAIRA BIANCHI; CESAR DE ANDRADE, ANDRE FURUGEN; DE ARRUDA, RUBENS PAES; BATISSACO, LEONARDO; FLOREZ-RODRIGUEZ, SHIRLEY ANDREA; MARTINS DE OLIVEIRA, BRUNA MARCELE; TORRES, MARIANA ANDRADE; LANCONI, RENATA; RAVAGNANI, GISELE MOURO; DO PRADO FILHO, ROBERTO ROMANO; et al. Recovery of normal testicular temperature after scrotal heat stress in rams assessed by infrared thermography and its effects on seminal characteristics and testosterone blood serum concentration. Theriogenology, v. 86, n. 3, p. 795-805, AUG 2016. (12/00040-0, 11/16744-3)

RODRIGUES ALVES, MAIRA BIANCHI; DE ARRUDA, RUBENS PAES; BATISSACO, LEONARDO; FLOREZ-RODRIGUEZ, SHIRLEY ANDREA; MARTINS DE OLIVEIRA, BRUNA MARCELE; TORRES, MARIANA ANDRADE; RAVAGNANI, GISELE MOURO; LANCONI, RENATA; DE ALMEIDA, TAMIE GUIBU; STORILLO, VANESSA MARTINS; et al. Low-level laser therapy to recovery testicular degeneration in rams: effects on seminal characteristics, scrotal temperature, plasma testosterone concentration, and testes histopathology. Lasers in Medical Science, v. 31, n. 4, p. 695-704, MAY 2016. (12/00040-0, 11/16744-3)

DE ALMEIDA, TAMIE GUIBU; RODRIGUES ALVES, MAIRA BIANCHI; BATISSACO, LEONARDO; TORRES, MARIANA ANDRADE; CESAR DE ANDRADE, ANDRE FURUGEN; MINGOTI, RODOLFO DANIEL; DE ARRUDA, RUBENS PAES; CARVALHO CELEGHINI, ENEIVA CARLA. Does low-level laser therapy on degenerated ovine testes improve post-thawed sperm characteristics?. Lasers in Medical Science, v. 34, n. 5, p. 1001-1009, JUL 2019. (13/13438-4, 12/00040-0)

BATISSACO, LEONARDO; DE ARRUDA, RUBENS PAES; RODRIGUES ALVES, MAIRA BIANCHI; TORRES, MARIANA ANDRADE; LEMES, KLEBER MENEGON; PRADO-FILHO, ROBERTO ROMANO; DE ALMEIDA, TAMIE GUIBU; CESAR DE ANDRADE, ANDRE FURUGEN; CARVALHO CELEGHINI, ENEIVA CARLA. Cholesterol-loaded cyclodextrin is efficient in preserving sperm quality of cryopreserved ram semen with low freezability. REPRODUCTIVE BIOLOGY, v. 20, n. 1, p. 14-24, MAR 2020. (12/00040-0, 12/09927-7, 13/13438-4)