Gene expression study: identification of relevant genes in development of the anterior sholuder instability

• Grant number: 11/10033-8
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2012
• End date: April 30, 2014
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Moises Cohen
• Grantee: Moises Cohen
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Mariana Ferreira Leal ; Marilia de Arruda Cardoso Smith ; Síntia Iole Nogueira Belangero

Abstract

The anterior shoulder dislocation occurs in about 2% of the population in which 80% of cases affect young individuals. The recurrent instability after the first episode of anterior dislocation is quite often being reported in high-risk populations, reaching 100% of cases. It is a highly disabling disease with major impact on quality of life of patients, it affects mostly young people in the productive phase, represents a considerable economic harm to public health. Its etiology is complex and multifactorial, and associated with genetic and environmental etiological factors. The objectives of this study are: a) to evaluate patients with traumatic anterior shoulder instability and the profile of expression of genes encoding type I collagen (COL1A1, COL1A2), type III (COL3A1) and type V (COL5A1), comparing different regions of the shoulder joint capsule (affected and not affected); b) correlate genetic findings with clinical data of the patient to a better understanding of disease pathogenesis. Thirty patients with traumatic anterior shoulder instability will be evaluated, recruited in the service of Orthopedics and Traumatology, Hospital São Paulo. The gene expression studies will be performed by the technique of Real Time PCR with Taqman ® detection system and will be used as a method of analysis the Comparative Ct. We expect to find at least one gene differentially expressed between the damaged tissue and healthy tissue, so it can be used as a marker for this type of injury. We hope also that the results may help in understanding how genetic changes may predispose to these lesions. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PAULO SANTORO BELANGERO; MARIANA FERREIRA LEAL; ALBERTO DE CASTRO POCHINI; CARLOS VICENTE ANDREOLI; BENNO EJNISMAN; MOISES COHEN. Perfil de expressão de genes do colágeno na cápsula glenoumeral de pacientes com instabilidade traumática anterior do ombro. Rev. bras. ortop., v. 49, n. 6, p. 642-646, 2014-12-00. (11/10033-8)