Articular cartilage repair with Type II collagen scaffold and adipose derived mesenchimal stem cells: an experimental sheep model study

• Grant number: 11/17596-8
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2012
• End date: June 30, 2014
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: João Batista de Miranda
• Grantee: João Batista de Miranda
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The function of articular cartilage in synovial joints is to absorb and distribute compression and shear forces transmitted by articulating bones. Because it is a vessel-free tissue, it has a low capacity for regeneration. Several conditions can cause its injury which can cause chronic pain, functional disability and osteoarthritis. Current methods for the treatment of condral lesions have significant limitations. The development of cell culture techniques and spongy matrix that can support these cells has become a promising approach. The objective of this project is to evaluate the ability of mesenchymal stem cells derived from human adipose tissue, cultured in a three-dimensional porous solid matrix made of collagen type II, to produce hyaline cartilage in a large animal model of chondral lesion. The same method has been successfully employed by the same group in small animals (rabbits). Fifteen sheep will be used. In each knee will be created a defect of seven millimeters in diameter in the loading area of the medial femoral condyle, resulting in 30 defects. Will be formed three groups treated with one of the following: type II collagen implant empty, type II collagen implant filled with mesenchymal cells from human fat, controls left empty. The animals will be sacrificed after six months following ethical protocols previously approved by the Ethics Committee on Animal Use (CEUA). Histological sections will be obtained for completion of the ICRS 2 index, O'Driscoll index and evaluation of the three dimensional structure of the extracellular matrix in polarized light microscopy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZORZI, ALESSANDRO R.; AMSTALDEN, ELIANE M. I.; PLEPIS, ANA MARIA G.; MARTINS, VIRGINIA C. A.; FERRETTI, MARIO; ANTONIOLI, ELIANE; DUARTE, ADRIANA S. S.; LUZO, ANGELA C. M.; MIRANDA, JOAO B.. Effect of Human Adipose Tissue Mesenchymal Stem Cells on the Regeneration of Ovine Articular Cartilage. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 16, n. 11, p. 26813-26831, NOV 2015. (11/17596-8)

ZORZI, ALESSANDRO R.; AMSTALDEN, ELIANE M. I.; PLEPIS, ANA MARIA G.; MARTINS, VIRGINIA C. A.; FERRETTI, MARIO; ANTONIOLI, ELIANE; DUARTE, ADRIANA S. S.; LUZO, ANGELA C. M.; MIRANDA, JOAO B.. Effect of Human Adipose Tissue Mesenchymal Stem Cells on the Regeneration of Ovine Articular Cartilage. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 16, n. 11, p. 19-pg., 2015-11-01. (11/17596-8)