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Onlay bone grafts fixation and integration with N-butil-2-cyanoacrilate, positional screws and lag screws: a histological, micro-CT and biomolecular study in rabbits


The use of titanium screws is the most currently used fixation method for fixation of "onlay" bone grafts. However, several disadvantages have been associated with this technique, such as the production of artifacts in CT scans and magnetic resonance imaging, corrosion in the implanted site, allergic reactions, infections, screws lost, low temperature sensitivity and alveolar bone resorption caused by a phenomenon known as "stress shielding". The Lag Screw technique is routinely used in jaws and has the advantage of maximum stability when compared to other fixation techniques. Because of the many drawbacks of the onlay bone grafting technique with the use of titanium screws or Lag Screws for bone grafts fixation, alternative techniques of graft fixation have been sought. One such technique is the cyanoacrylate-based adhesive. When it's used for bone fixation, the cyanoacrylate glue has some potential advantages over the use of metal and resorbable screws, making it a possible alternative to conventional means of osteosynthesis. This includes: low cost, simple technique and allows modification of the graft fixation position during the surgical procedure. This study aims to: (1) analyze bone grafts volume changes obtained from the skull and attached to the rabbits' jaw with cyanoacrylate-based adhesive (NB-Cn), conventional screw, Lag Screw technique or different combinations, (2) histologically analyze the dynamics of incorporation of the graft to the recipient bone bed, (3) evaluate changes in volume and density of the grafts using imaging obtained by Micro-CT scans, (4) expressions of genes IL-6, IL-10, VEGF, RANKL, OPG, MEPE, TNF-±, cathepsin K, TRAP, BMP-2, ALP, osteocalcin, and Runx2 osterix SP7-related remodeling of the grafts subjected to different fixation procedures by RT-PCR. Each group will consist of 08 (eight) animals, which will be sacrificed in order to analyze the different types of fixation in 3rd, 7th, 20th and 40th days after surgery. The data obtained in this study may contribute to a better understanding on the effects of cyanoacrylates, providing important tools for the development of new techniques for fixation of bone grafts. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CANEVA, MARCO; BOTTICELLI, DANIELE; CARNEIRO MARTINS, EVANDRO NETO; CANEVA, MARTINA; LANG, NIKLAUS P.; XAVIER, SAMUEL P. Healing at the interface between recipient sites and autologous block bone grafts affixed by either position or lag screw methods: a histomorphometric study in rabbits. Clinical Oral Implants Research, v. 28, n. 12, p. 1484-1491, DEC 2017. Web of Science Citations: 3.
DE SANTIS, ENZO; SILVA, ERICK RICARDO; CARNEIRO MARTINS, EVANDRO NETO; FAVERO, RICCARDO; BOTTICELLI, DANIELE; XAVIER, SAMUEL PORFIRIO. Healing at the Interface Between Autologous Block Bone Grafts and Recipient Sites Using n-Butyl-2-Cyanoacrylate Adhesive as Fixation: Histomorphometric Study in Rabbits. JOURNAL OF ORAL IMPLANTOLOGY, v. 43, n. 6, p. 447-455, DEC 2017. Web of Science Citations: 2.
SALATA, LUIZ A.; MARIGUELA, VIVIANE C.; ANTUNES, ANTONIO A.; GROSSI-OLIVEIRA, GUSTAVO; ALMEIDA, ADRIANA; TABA, JR., MARIO. Short-Term Evaluation of Grafts Fixed With Either N-Butyl-2-Cyanocrylate or Screws. JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, v. 72, n. 4, p. 676-682, APR 2014. Web of Science Citations: 7.

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