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Hydrogen bio-production in anaerobic batch reactor for low substrate concentrations


The environmental pollution has grown rapidly, mainly due to urbanization and industrialization. Cleaner energy sources have been sought in order to satisfy the growing world energy demand. The hydrogen gas produced in wastewater treatment through biological processes can be used as an alternative energy source, combining not only biological treatment of wastewater, but also the achievement of sustainable energy. The aim of this study will be obtain biological production of hydrogen gas from low concentrations of substrates and similar to those contained in wastewater industries of soft drinks, beer and vinasse, identify and characterize the microbial diversity responsible for hydrogen production in anaerobic batch reactors. The experiments will be mounted in anaerobic batch reactors, in triplicate, with headspace filled with helium (100%) at 37 ° C in order to obtain the anaerobic cultures hydrogen producers. Glucose, sucrose and xylose are the substrates used in small concentrations and close to industrial effluents. The inoculum used will be the granular sludge from the UASB reactor used in the treatment of industrial wastewater from the Heinkein brewery (Araraquara - SP). The purification of microorganisms will be held from the serial dilutions on synthetic substrate and the determination of the kinetics of growth and hydrogen generation in batch reactors. The physical-chemical and chromatographic analyses related to the consumption of substrate, generation of hydrogen and other intermediates products such as alcohols and volatile acids will be held. The microbiological and molecular biology techniques such as cloning and sequencing of 16S ribosomal DNA fragments will be used to identify organisms responsible for hydrogen production. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE MOURA TORQUATO, LILIAN DANIELLE; DE ALMEIDA, SONIA; DE OLIVEIRA, JOSE EDUARDO; CRESPI, MARISA SPIRANDELI; MAINTINGUER, SANDRA IMACULADA. Thermal characterization of anaerobic sludges from wastewater treatments applied to biological generation of H-2. JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY, v. 127, n. 2, p. 1267-1275, FEB 2017. Web of Science Citations: 3.
MAINTINGUER, SANDRA IMACULADA; LAZARO, CAROLINA ZAMPOL; PACHIEGA, RENAN; AMANCIO VARESCHE, MARIA BERNADETE; SEQUINEL, RODRIGO; DE OLIVEIRA, JOSE EDUARDO. Hydrogen bioproduction with Enterobacter sp isolated from brewery wastewater. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v. 42, n. 1, p. 152-160, JAN 5 2017. Web of Science Citations: 11.
RODRIGUES, CAROLINE VARELLA; SANTANA, KAMILI OLIVEIRA; NESPECA, MAURILIO GUSTAVO; DE OLIVEIRA, JOSE EDUARDO; MAINTINGUER, SANDRA IMACULADA. Crude glycerol by transesterification process from used cooking oils: Characterization and potentialities on hydrogen bioproduction. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v. 41, n. 33, p. 14641-14651, SEP 7 2016. Web of Science Citations: 11.

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