| Grant number: | 12/01318-1 |
| Support Opportunities: | Regular Research Grants |
| Start date: | May 01, 2012 |
| End date: | April 30, 2014 |
| Field of knowledge: | Engineering - Sanitary Engineering - Water Supply and Wastewater Treatment |
| Principal Investigator: | Sandra Imaculada Maintinguer |
| Grantee: | Sandra Imaculada Maintinguer |
| Host Institution: | Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil |
| Associated researchers: | Alberto Jose Cavalheiro ; Angela Regina Araújo ; Maria Bernadete Amâncio Varesche |
Abstract
The environmental pollution has grown rapidly, mainly due to urbanization and industrialization. Cleaner energy sources have been sought in order to satisfy the growing world energy demand. The hydrogen gas produced in wastewater treatment through biological processes can be used as an alternative energy source, combining not only biological treatment of wastewater, but also the achievement of sustainable energy. The aim of this study will be obtain biological production of hydrogen gas from low concentrations of substrates and similar to those contained in wastewater industries of soft drinks, beer and vinasse, identify and characterize the microbial diversity responsible for hydrogen production in anaerobic batch reactors. The experiments will be mounted in anaerobic batch reactors, in triplicate, with headspace filled with helium (100%) at 37 ° C in order to obtain the anaerobic cultures hydrogen producers. Glucose, sucrose and xylose are the substrates used in small concentrations and close to industrial effluents. The inoculum used will be the granular sludge from the UASB reactor used in the treatment of industrial wastewater from the Heinkein brewery (Araraquara - SP). The purification of microorganisms will be held from the serial dilutions on synthetic substrate and the determination of the kinetics of growth and hydrogen generation in batch reactors. The physical-chemical and chromatographic analyses related to the consumption of substrate, generation of hydrogen and other intermediates products such as alcohols and volatile acids will be held. The microbiological and molecular biology techniques such as cloning and sequencing of 16S ribosomal DNA fragments will be used to identify organisms responsible for hydrogen production. (AU)
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