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Hydrogen Bio-production to low concentrations of fructose and glucose.

Grant number: 13/03815-5
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2013
Effective date (End): January 31, 2014
Field of knowledge:Engineering - Sanitary Engineering - Water Supply and Wastewater Treatment
Principal Investigator:Sandra Imaculada Maintinguer
Grantee:Mayara Franco Rodrigues
Home Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Cleaner energy sources have been pursued in order to meet the growing global energy demand. The hydrogen gas produced in the wastewater treatment by means of biological processes can be used as an alternative energy source, combining not only the biological treatment of the effluent, but also to achieve sustainable energy. The aim of this work is to obtain biological production of hydrogen gas from low concentrations of fructose and glucose similar to those contained in wastewater from citrus industries in the region and characterizes the bacterial consortium responsible for hydrogen production in anaerobic batch. The experiments will be assembled in triplicate, with headspace filled with nitrogen (100%), at 37 ° C, in order to obtain anaerobic cultures producing hydrogen. The inoculum used is granular sludge from upflow anaerobic acidogenic and upflow sludge blanket (UASB) from industrial effluent, previously purified. Physical-chemical and chromatographic analysis related to the consumption of the substrate, hydrogen generation and other intermediate products such as alcohols and volatile acids will be held. Microbiological examination as phase contrast microscopy, light common Gram stain and endospore be used to characterize the morphologies of the microorganisms responsible for the hydrogen bio-production.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PACHIEGA, RENAN; RODRIGUES, MAYARA FRANCO; RODRIGUES, CAROLINE VARELLA; SAKAMOTO, ISABEL KIMIKO; VARESCHE, MARIA BERNADETE A.; DE OLIVEIRA, JOSE EDUARDO; MAINTINGUER, SANDRA IMACULADA. Hydrogen bioproduction with anaerobic bacteria consortium from brewery wastewater. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v. 44, n. 1, SI, p. 155-163, JAN 1 2019. Web of Science Citations: 5.

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