Studies of effect of serineproteinase inhibitors from Rhipicephalus Boophilus microplus tick into lung emphysema induced in mice and molecular characterization of serineproteinases activities present in its experimental model

• Grant number: 11/07001-7
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2012
• End date: October 31, 2014
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Sergio Daishi Sasaki
• Grantee: Sergio Daishi Sasaki
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil

Abstract

Chronic obstructive pulmonary disease (COPD) can be described as an assembly of pathological modifications, since chronic inflammation up to pulmonary tissue degradation caused by proteinases. At the present moment there are not specific medicines to treat the disease. The most commonly problems in COPD are chronic bronchitis and emphysema, which are the main causes of mortality in adults and elder people. The metalloproteinases and serine proteinases, specifically human neutrophil elastase, are involved in the first steps of disease development. Despite of treatments used to avoid the advance of its disease to more serious stages, its considered irreversible yet, and generally it takes to development of others cardio respiratory problems and to death. Serine proteinase inhibitors are considered as one possible alternative treatment against emphysema to avoid the development of disease, although there is not any definitive therapy using these molecules until now. In this work we propose to investigate the potential of the serine proteinases inhibitors rBmTI-A and rBmTI-6 as protective molecules against lung emphysema development in mice model, additionally we will verify the production of serine proteinases in this model by activities assays and microarray. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, GRAZIELE CRISTINA; ALVES DURAN, ADRIANA FELICIANO; SANTOS DA SILVA, FLAVIA RIBEIRO; BOMEDIANO, LIVIA DE MORAES; MACHADO, GABRIEL CAPELLA; SASAKI, SERGIO DAISHI. Neutrophil elastase inhibitor purification strategy from cowpea seeds. PLoS One, v. 14, n. 10, OCT 10 2019. (11/07001-7)

BOMEDIANO CAMILLO, LIVIA DE MORAES; FERREIRA, GRAZIELE CRISTINA; ALVES DURAN, ADRIANA FELICIANO; SANTOS DA SILVA, FLAVIA RIBEIRO; GARCIA, WANIUS; SCOTT, ANA LIGIA; SASAKI, SERGIO DAISHI. Structural modelling and thermostability of a serine protease inhibitor belonging to the Kunitz-BPTI family from the Rhipicephalus microplus tick. Biochimie, v. 181, p. 226-233, FEB 2021. (11/07001-7, 17/19077-4, 18/11874-5, 17/17275-3)

ALVES DURAN, ADRIANA FELICIANO; NEVES, LUANA DE PAIVA; SANTOS DA SILVA, FLAVIA RIBEIRO; MACHADO, GABRIEL CAPELLA; FERREIRA, GRAZIELE CRISTINA; LOURENCO, JULIANA D.; TANAKA, APARECIDA SADAE; MARTINS, MILTON DE ARRUDA; LOPES, FERNANDA D. T. Q. S.; SASAKI, SERGIO DAISHI. rBmTI-6 attenuates pathophysiological and inflammatory parameters of induced emphysema in mice. International Journal of Biological Macromolecules, v. 111, p. 1214-1221, MAY 2018. (11/07001-7)