Storage lesion and interleukines concentration in packed red blood cells collected on CPDA-1 and CPD-SagM, with and without leucoreducer filter

• Grant number: 12/00375-1
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2012
• End date: February 28, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Denise Tabacchi Fantoni
• Grantee: Denise Tabacchi Fantoni
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Transfusion medicine advocates the use of blood products because it allows increasing the shelf life of blood products and offering the most specific. For substances that are used with anticoagulant additives that lets you keep stock packed red blood cells (PRBC) for more than 24 hours. However, the handling and storage can lead to blood by products functional changes attending to damage to the receiver. Erythrocytes are anucleate cells whose metabolism still active during the storage period, with maintenance of lactate production, pH and consequently reducing their shelf life. Besides changing the erythrocyte membrane, the low pH hinders the rearrangement of 2,3 DPG molecule responsible for the control of hemoglobin affinity for oxygen, resulting in decreased oxygen delivery to tissues by red blood cells transfused. The presence of leukocytes courses with production of inflammatory mediators and interleukins that may be responsible for transfusion reactions, such as non-hemolytic febrile reactions and transfusion related acute lung injury. Therefore it is observed that, while necessary to maintain the stock of red cell components, storage blood can reduce the function of transfused red blood cells and cause transfusion reactions. This study aims to evaluate the production of cytokines and analyze the possible injuries storage canine PRBC collected with CPDA-1 and SAG-M in order to determine whether there are differences in the quality and potential of inflammatory blood products produced. (AU)