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Evaluation of the concentration of lactate, serum potassium and hemogasometry in dogs with anemia receiving transfusions of concentrated red blood cells with different storage periods

Grant number: 12/00338-9
Support Opportunities:Regular Research Grants
Duration: August 01, 2012 - July 31, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal Investigator:Aline Magalhães Ambrósio
Grantee:Aline Magalhães Ambrósio
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated researchers:Caroline Yumi Kayano ; Denise Tabacchi Fantoni ; Ludmila Rodrigues Moroz ; Renata Ramos Rodrigues


Storage of packed red blood cells (RBC) can result in biochemical changes in blood gas and blood transfusion which may induce the occurrence of clinical and laboratory variables in patients receiving. It is known that from 7 days of storage, can be observed increased levels of lactate and potassium and a pH decrease in blood bags of dogs. However, it is unclear whether similar changes also occur during storage of the canine and RBC is no difference between the clinical and laboratory dogs receiving RBC with different storage periods. Thus, the present study, in its first phase aims to evaluate only the pockets of RBC in different storage periods and as the second phase, to compare the concentrations of lactate and potassium and blood gas values in dogs with anemia who received stored RBC also for different periods. To do so, will be evaluated 21 adult dogs that will receive randomly RBC contained in bags with CPDA-1 stored at a temperature of 4-6 ° C for up to 7 days (group I, n = 7), RBC stored for 8 to 14 days (group II, n = 7) or RBC stored for 15 to 21 days (group III, n = 7). Will be evaluated and the concentrations of potassium lactate and blood gas values of the dogs immediately before transfusion, after the transfusion and 24 hours after transfusion of RBC. These same factors will also be measured in RBC immediately prior to administration. The data will be subjected to analysis of variance for repeated measures followed by Tukey test and Student t test. The degree of significance for statistical analysis is P <0.05. (AU)

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