Molecular characterization of avian pathogenic (APEC) and human uropathogenic (UPEC) Escherichia coli strains

• Grant number: 12/04931-6
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2012
• End date: August 31, 2014
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Wanderley Dias da Silveira
• Grantee: Wanderley Dias da Silveira
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Renato Pariz Maluta

Abstract

Brazil is the third larger producer and the larger exporter of poultry meat in the world. The production is estimated on over 12 millions of ton. From this amount, almost 4 millions of tons are exported, yielding an income of about US$ 6 billions. Lineages of avian pathogenic Escherichia coli (APEC) are agents of significant degrees of morbidity and mortality for meat poultry and layer hens, causing losses to the poultry industry. Furthermore, APEC lineages have recently been described as potential zoonotic agents. For several years, our group have worked on APEC lineages for characterizing them with respect to their biological characteristics related to patogenicity (resistance to antimicrobials, expression of fimbriae and flagella, colicinis, enterobactins, resistance to bactericidal serum effects, adhesion and invasion of cell lineages ability), genetics (existence of pathogenicity related genes in plasmids and chromosomes) and population analysis (clonal structure). In this project, we wish to study, in a comparative way, APEC lineages isolated in different Brazilian regions [South (Paraná and Rio Grande do Sul); South-East (São Paulo) and North-East (Pernambuco, Rio Grande do Norte and Paraíba)] with E. coli lineages isolated from human urinary tract infections, termed uropathogenic E. coli (UPEC), ir order to assess their clonal structure and whether or not APEC may act as a zoonotic agent. For this goal, it will be performed molecular techniques such as characterization by PCR of 43 genes, determination of pulsed field gel electrophoresis (PFGE) patterns and multilocus sequence typing (MLST) of each isolate in regard to their clonal characterization. Additionally, the isolates will be serogruped. All these data, when put together, will allow us to determine which type of APEC lineages are the most prevalent in our country and which are their genetic constitutions in regard to the presence of virulence genes. It could open the way for the establishement of representative strains for the development of vaccinal lineages. Furthermore, the comparison with UPEC lineages will show whether Brazilian APEC lineages present zoonotic potential similarly to what has been reported in lineages isolated in some other countries. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVEIRA, F.; MALUTA, R. P.; TIBA, M. R.; DE PAIVA, J. B.; GUASTALLI, E. A. L.; DA SILVEIRA, W. D.. Comparison between avian pathogenic (APEC) and avian faecal (AFEC) Escherichia coli isolated from different regions in Brazil. VETERINARY JOURNAL, v. 217, p. 65-67, NOV 2016. (12/04931-6, 12/05073-3)

MALUTA, RENATO PARIZ; LOGUE, CATHERINE MARY; TIBA CASAS, MONIQUE RIBEIRO; MENG, TING; LOPES GUASTALLI, ELISABETE APARECIDA; GALVAO ROJAS, THAIS CABRERA; MONTELLI, AUGUSTO CEZAR; SADATSUNE, TERUE; RAMOS, MARCELO DE CARVALHO; NOLAN, LISA KAY; et al. Overlapped Sequence Types (STs) and Serogroups of Avian Pathogenic (APEC) and Human Extra-Intestinal Pathogenic (ExPEC) Escherichia coli Isolated in Brazil. PLoS One, v. 9, n. 8, AUG 12 2014. (12/04931-6, 13/09167-5, 12/05073-3)

THAÍS C.G. ROJAS; RENATO P. MALUTA; LUCIANO V. KOENIGKAN; WANDERLEY DIAS DA SILVEIRA. Análises in silico da filogenia e do perfil de genes associados à virulência, dos genomas de linhagens de Escherichia coli de origem aviária. Pesquisa Veterinária Brasileira, v. 34, n. 2, p. 129-133, 2014-02-00. (12/04931-6)

MALUTA, RENATO PARIZ; VICCARI GATTI, MARIA SILVIA; JOAZEIRO, PAULO PINTO; DE PAIVA, JACQUELINE BOLDRIN; GALVAO ROJAS, THAIS CABRERA; SILVEIRA, FLAVIO; HOULE, SEBASTIEN; TAKAYAMA KOBAYASHI, RENATA KATSUKO; DOZOIS, CHARLES M.; DA SILVEIRA, WANDERLEY DIAS. Avian Extraintestinal Escherichia coli Exhibits Enterotoxigenic-Like Activity in the In Vivo Rabbit Ligated Ileal Loop Assay. FOODBORNE PATHOGENS AND DISEASE, v. 11, n. 6, p. 484-489, JUN 2014. (12/04931-6, 12/05073-3)

MALUTA, RENATO PARIZ; LEITE, JANAINA LUISA; GALVAO ROJAS, THAIS CABRERA; AFFONSO SCALETSKY, ISABEL CRISTINA; LOPES GUASTALLI, ELISABETE APARECIDA; RAMOS, MARCELO DE CARVALHO; DA SILVEIRA, WANDERLEY DIAS. Variants of astA gene among extra-intestinal Escherichia coli of human and avian origin. FEMS Microbiology Letters, v. 364, n. 6, MAR 2017. (12/04931-6)

GALVAO ROJAS, THAIS CABRERA; LOBO, FRANCISCO PEREIRA; HONGO, JORGE AUGUSTO; VICENTINI, RENATO; VERMA, RENU; MALUTA, RENATO PARIZ; DA SILVEIRA, WANDERLEY DIAS. Genome-Wide Survey of Genes Under Positive Selection in Avian Pathogenic Escherichia coli Strains. FOODBORNE PATHOGENS AND DISEASE, v. 14, n. 5, p. 245-252, MAY 2017. (12/04931-6)

GALVAO ROJAS, THAIS CABRERA; MALUTA, RENATO PARIZ; PARIZZI, LUCAS PEDERSEN; KOENIGKAN, LUCIANO VIEIRA; YANG, JIAN; YU, JUN; GUIMARAES PEREIRA, GONCALO AMARANTE; DA SILVEIRA, WANDERLEY DIAS. Genome Sequences of Avian Pathogenic Escherichia coli Strains Isolated from Brazilian Commercial Poultry. GENOME ANNOUNCEMENTS, v. 1, n. 2, p. 2-pg., 2013-03-01. (12/04931-6, 12/05073-3)