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Optimization of methods using mass spectrometry to protein complex mixture analysis

Grant number: 11/07907-6
Support type:Research Grants - Visiting Researcher Grant - International
Duration: October 29, 2011 - November 22, 2011
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Adriana Franco Paes Leme
Grantee:Adriana Franco Paes Leme
Visiting researcher: Nicholas Eugene Sherman
Visiting researcher institution: University of Virginia (UVa), United States
Home Institution: Laboratório Nacional de Luz Síncrotron (LNLS). Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia, Inovações e Comunicações (Brasil). Campinas , SP, Brazil


During October 29 to November 22, Dr. Nicholas Sherman from University of Virginia will participate of activities to the development and optimization of methods in Mass Spectrometry Laboratory at LNBio. In addition, he will be a speaker in the Proteomics Use's Meeting to be held at LNBio in November. Twenty internal and external users will be selected for this meeting. Among the activities, Dr. Nicholas will participate in experiments with protein complex mixtures, endogenous peptide analysis, phosphoprylated peptide analysis, discussions about isotopic labeling, Proteome Discoverer Software and instrumentation (the details are attached). Methodologies that have been used in the most important international groups of proteomic and mass spectrometry and that are being used by Dr. Nicholas in his laboratory will be optimized at LNBio. For these experiments we will also use the LTQ Velos Orbitrap, approved by Programa Multiusuário 3 of FAPESP. (AU)