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Proteomic studies in Peperomia obtusifolia (Piperaceae)

Grant number: 11/01003-8
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): July 01, 2011
Effective date (End): June 30, 2016
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Maysa Furlan
Grantee:Andrea Nastri de Luca Batista
Home Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated research grant:13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery, AP.CEPID
Associated scholarship(s):12/16484-4 - Raman optical activity: an innovative tool for protein structural analysis, BE.EP.PD

Abstract

Bioguided phytochemical studies in Peperomia obtusifolia led to the isolation of a series of bioactive secondary metabolites, including prenylated chromanes, lignans, amides, flavonoids and other phenolic derivatives. The prenylated chromanes showed potent trypanocidal activity. Based on a literature survey, the prenylated compounds seem to be responsible for most of the biological activities reported in Peperomia. Additionally, the prenylated benzopyrans were isolated as racemic and/or diastereomeric mixtures, which raises questions about the level of stereochemical control in the addition of prenyl units as well as the cyclization and formation of the 3,4-dihydro-2H-pyran ring. Thus, this project aims to identify the protein profile of this species and the consequent development of proteomic reference maps, complementing previous results regarding the in vitro activity of oxidoreductases, PAL, prenyl- and geranyltransferases in Piperaceae species. The proteomics analysis will be carried out in three main steps: extraction, analysis and identification of proteins. The extraction of the protein complex will be performed based on protocols already established by the research group headed by Prof. Furlan. The structural analysis and the identification of proteins will be made by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MALDI-TOF or ESI-MS / MS). Chromatographic techniques such as ion chromatography using adsorbents, gel filtration and electrophoresis will be used for the fractionation and purification of the proteins of interest. The secondary structure of isolated proteins will be determined by using vibrational circular dichroism (VCD) spectroscopy.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BATISTA, ANDREA N. L.; BATISTA, JR., JOAO M.; SOUZA-MOREIRA, TATIANA M.; VALENTINI, SANDRO R.; KATO, MASSUO J.; ZANELLI, CLESLEI F.; FURLAN, MAYSA. Biosynthetic Insights into p-Hydroxybenzoic Acid-Derived Benzopyrans in Piper gaudichaudianum. Journal of the Brazilian Chemical Society, v. 29, n. 5, p. 1105-1114, MAY 2018. Web of Science Citations: 0.
BATISTA, ANDREA N. L.; DOS SANTOS-PINTO, JOSE ROBERTO A.; BATISTA, JR., JOAO M.; SOUZA-MOREIRA, TATIANA M.; SANTONI, MARIANA M.; ZANELLI, CLESLEI F.; KATO, MASSUO J.; LOPEZ, SILVIA N.; PALMA, MARIO S.; FURLAN, MAYSA. The Combined Use of Proteomics and Transcriptomics Reveals a Complex Secondary Metabolite Network in Peperomia obtusifolia. Journal of Natural Products, v. 80, n. 5, p. 1275-1286, MAY 2017. Web of Science Citations: 4.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.