Treatment of subclinical mastitis in sheep during the dry period with conventional and nanoparticle antimicrobials

• Grant number: 12/03847-1
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2012
• End date: July 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Luiz Francisco Zafalon
• Grantee: Luiz Francisco Zafalon
• Host Institution: Embrapa Pecuária Sudeste. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil

Abstract

The mastitis is considered an obstacle to the sheep productiom due to economic losses. Methods of control for the disease in Brazilian herds are little studied and there are no treatment protocols appropriate to the ovine species. The purpose os this study is to establish treatment protocol against subclinical mastitis on the basis of intramammary infusion of antimicrobial active ingredient in time of drying of meat sheep, in different dosages. The efficiency of cloxacillin will be determined for microbiological cure of subclinical mastitis, in two different dosages in conventional form, 600 mg and 100 mg, and in the nanoparticulate form (100 mg). The experiment will be carried out in an experimental herd, using 75 sheep. Clinical examinations will be performed in the structures of teats and milk samples will be collected for diagnosis of subclinical mastitis by using California Mastitis Test (CMT) and somatic cell count (SCC). The confirmation of the results will be performed with the use of microbiological culture before and after the treatments, which will be distributed in the following experimental groups: G1 (positive control); G2 (negative control); G3 (infusion on 600 mg of cloxacillin); G4 (infusion of 100 mg of cloxacillin); G5 (100 mg of cloxacillin nanoparticulate). The results will be analyzed using Chi-square test and Fisher's exact test, in dependence of the values found, to compare cure rates in different groups. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZAFALON, LUIZ FRANCISCO; MASCARENHAS SANTANA, RAUL COSTA; PILON, LUCAS EDUARDO; FIM JUNIOR, GUILHERME APARECIDO. Diagnosis of subclinical mastitis in Santa Ins and Morada Nova sheep in southeastern Brazil. TROPICAL ANIMAL HEALTH AND PRODUCTION, v. 48, n. 5, p. 967-972, JUN 2016. (12/03847-1)

RAUL C.M. SANTANA; LUIZ FRANCISCO ZAFALON; HUMBERTO DE M. BRANDÃO; GUILHERME A.F. JUNIOR; LUCAS EDUARDO PILON; WALDOMIRO B. JUNIOR; RODRIGO GIGLIOTI; VANESSA CARLA F. MOSQUEIRA. Uso de antimicrobiano nanoparticulado para o tratamento da mastite subclínica de ovelhas de corte no período seco. Pesquisa Veterinária Brasileira, v. 36, n. 9, p. 826-830, 2016-09-00. (12/03847-1)

ZAFALON, LUIZ FRANCISCO; FIM JUNIOR, GUILHERME APARECIDO; VASO, CAROLINA ORLANDO; DOS SANTOS LOPES, NAHRYDA SAMARA; APARECIDA VESCHI, JOSIR LAINE; MASCARENHAS SANTANA, RAUL COSTA. Influência dos estágios de lactação e ocorrência de chuvas sobre a mastite subclínica em ovelhas com aptidão para produção de carne. Ciência Rural, v. 46, n. 10, p. 1797-1803, OCT 2016. (12/03847-1)