Cardiovascular regulation under physiological pathophysiological conditions

• Grant number: 09/50389-6
• Support Opportunities: Research Projects - Thematic Grants
• Duration: July 01, 2009 - June 30, 2013
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Helio Cesar Salgado
• Grantee: Helio Cesar Salgado
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Pesquisadores principais: Maria Cristina de Oliveira Salgado ; Valéria Paula Sassoli Fazan

Abstract

The present study was grouped into five projects. In projects 1 it will be investigated the post excitatory depression (PED) of the aortic depressor nerve (ADN) after electrical stimulation of the baroreceptors afferents. In this project it will be examined, particularly, the role played by autocrine/paracrine factors (PGI2 e ROS) in the development of PED. In projects 2 a technique for immunohistochemical staining for substance P in the ADN of normotensive rats will be developed and standardized, followed by the quantification of the amount of substance P in myelinated fibers. The techniques for staining substance P will be standardized for the exam of unmyelinated fibers by means of transmission electronic microscopy. In projects 3 it will be examined the hemodynamic responses to electrical stimulation of the aortic depressor nerve of conscious L-NAME hypertensive rats, submitted to the pharmacological blockade with methyl atropine, atenolol and prazosin. These protocols aim to evaluate the relative role played by autonomic afferences (sympathetic and parasympathetic) in arterial pressure and heart rate regulation in the L-NAME hypertensive model. In addition, it will be evaluated the responses of mesenteric and hindquarter resistance in the control of blood flow during the electrical stimulation of the ADN, by means of the Doppler flow meter. In projects 4 it will evaluated in conscious rats, during the development (2, 7 and 14 days) of L-NAME hypertension, the renal sympathetic nerve activity by means of telemetry. Finally, in projects 5 it will be investigated in rats and mice with chronic heart failure developed after coronary artery ligation, the chronic effect of acetylcholinesterase inhibition with pyridostigmine on autonomic balance, heart rate and arterial pressure variability in time (variance) and frequency domain (spectral analysis). In addition, it will be also investigated the reflex control of heart rate by means of the evaluation of spontaneous sensitivity of the baroreflex using the sequence method. The studies of cardiovascular variability in mice will be undertaken using telemetry. Still in projects 5 it will be evaluated in anesthetized rats and mice the influence of chronic acetylcholinesterase inhibition with pyridostigmine upon the cardiac function by means of the pressure vs. volume curve of the left ventricle. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORREA, WESLEY G.; DURAND, MARINA T.; BECARI, CHRISTIANE; TEZINI, GEISA C. S. V.; DO CARMO, JUSSARA M.; DE OLIVEIRA, MAURO; PRADO, CIBELE M.; FAZAN, JR., RUBENS; SALGADO, HELIO C.. Pyridostigmine prevents haemodynamic alterations but does not affect their nycthemeral oscillations in infarcted mice. AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL, v. 187, p. 50-55, JAN 2015. (09/50389-6)

OLIVEIRA, FABRICIO S.; NESSLER, RANDY A.; CASTANIA, JACI A.; SALGADO, HELIO C.; FAZAN, VALERIA P. S.. Ultrastructural and morphometric alterations in the aortic depressor nerve of rats due to long term experimental diabetes: Effects of insulin treatment. Brain Research, v. 1491, p. 197-203, JAN 23 2013. (10/12518-6, 09/50389-6, 09/16748-9)

SANADA, LUCIANA SAYURI; DA ROCHA KALIL, ANDREA LURDES; TAVARES, MARCELO RODRIGO; MAIA NEUBERN, MILENA CARDOSO; SALGADO, HELIO CESAR; SASSOLI FAZAN, VALERIA PAULA. Sural nerve involvement in experimental hypertension: morphology and morphometry in male and female normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). BMC NEUROSCIENCE, v. 13, MAR 2 2012. (10/12518-6, 09/50389-6, 09/16748-9)

SANADA, LUCIANA SAYURI; TAVARES, MARCELO RODRIGO; MAIA NEUBERN, MILENA CARDOSO; SALGADO, HELIO CESAR; SASSOLI FAZAN, VALERIA PAULA. Ratos Wistar podem ser utilizados como os controles normotensos dos ratos espontaneamente hipertensos (SHR) em investigações morfométricas de nervos periféricos?. Acta Cirurgica Brasileira, v. 26, n. 6, p. 514-520, NOV-DEC 2011. (09/16748-9, 09/50389-6, 10/12518-6)

CARVALHO DE VASCONCELOS, CARLOS AUGUSTO; SASSOLI FAZAN, VALERIA PAULA; MOORE, KENNETH CHARLES; NESSLER, RANDY ALAN; VALENCA, MARCELO MORAES. Transmission Electron Microscopy Studies of the Vestibulocochlear Nerve in Chronic Diabetic Rats. INTERNATIONAL JOURNAL OF MORPHOLOGY, v. 29, n. 1, p. 272-277, MAR 2011. (06/06362-8, 09/50389-6)

DA COSTA BILEGO NETO, ANTONIO PAULO; CASSIANO SILVEIRA, FERNANDO BRAGA; RODRIGUES DA SILVA, GREICE ANNE; SANADA, LUCIANA SAYURI; SASSOLI FAZAN, VALERIA PAULA. Reproducibility in Nerve Morphometry: Comparison between Methods and among Observers. BIOMED RESEARCH INTERNATIONAL, 2013. (10/12518-6, 09/50389-6)

DA COSTA BILEGO NETO, ANTONIO PAULO; CASSIANO SILVEIRA, FERNANDO BRAGA; RODRIGUES DA SILVA, GREICE ANNE; SANADA, LUCIANA SAYURI; SASSOLI FAZAN, VALERIA PAULA. Reproducibility in Nerve Morphometry: Comparison between Methods and among Observers. BIOMED RESEARCH INTERNATIONAL, v. 2013, p. 7-pg., 2013-01-01. (10/12518-6, 09/50389-6)

SATO, KARINA LAURENTI; SANADA, LUCIANA SAYURI; FERREIRA, RENATA DA SILVA; DE BARROS OLIVETI DE MARCO, MARIA CAROLINA DEL BEM; CASTANIA, JACI AIRTON; SALGADO, HELIO CESAR; NESSLER, RANDY ALAN; SASSOLI FAZAN, VALERIA PAULA. Renal nerve ultrastructural alterations in short term and long term experimental diabetes. BMC NEUROSCIENCE, v. 15, JAN 5 2014. (10/12518-6, 09/50389-6, 09/16748-9)

RODRIGUES, A. R.; FERREIRA, R. S.; SALGADO, H. C.; FAZAN, V. P. S.. Morphometric analysis of the phrenic nerve in male and female Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). Brazilian Journal of Medical and Biological Research, v. 44, n. 6, p. 583-591, JUN 2011. (09/50389-6)