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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Morphometric analysis of the phrenic nerve in male and female Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR)

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Author(s):
Rodrigues, A. R. [1] ; Ferreira, R. S. [1] ; Salgado, H. C. [2] ; Fazan, V. P. S. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Neurociencias & Ciencias Comportamento, BR-14049900 Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Fisiol, BR-14049900 Ribeirao Preto - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 44, n. 6, p. 583-591, JUN 2011.
Web of Science Citations: 6
Abstract

Ventilatory differences between rat strains and genders have been described but the morphology of the phrenic nerve has not been investigated in spontaneously hypertensive (SHR) and normotensive Wistar-Kyoto (WKY) rats. A descriptive and morphometric study of the phrenic nerves of male (N = 8) and female (N = 9) SHR, and male (N = 5) and female (N = 6) WKY is presented. After arterial pressure and heart rate recordings, the phrenic nerves of 20-week-old animals were prepared for epoxy resin embedding and light microscopy. Morphometric analysis was performed with the aid of computer software that took into consideration the fascicle area and diameter, as well as myelinated fiber profile and Schwann cell nucleus number per area. Phrenic nerves were generally larger in males than in females of both strains but larger in WKY compared to SHR for both genders. Myelinated fiber numbers (male SHR = 228 +/- 13; female SHR = 258 +/- 4; male WKY = 382 +/- 23; female WKY = 442 +/- 11 for proximal right segments) and density (N/mm(2); male SHR = 7048 +/- 537; female SHR = 10355 +/- 359; male WKY = 9454 +/- 1437; female WKY = 14361 +/- 1448 for proximal right segments) were significantly larger in females of both groups and remarkably larger in WKY than SHR for both genders. Strain and gender differences in phrenic nerve myelinated fiber number are described for the first time in this experimental model of hypertension, indicating the need for thorough functional studies of this nerve in male and female SHR. (AU)

FAPESP's process: 09/50389-6 - Cardiovascular regulation under physiological pathophysiological conditions
Grantee:Helio Cesar Salgado
Support Opportunities: Research Projects - Thematic Grants