Influence of pulpal infection associated with diabetes in the blood profile and, quantification of proinflammatory cytokines TNF-±, IL-6, IL-17. Microscopic analysis, blood measurement and study in organs of rats.

• Grant number: 12/02083-8
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2012
• End date: August 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Luciano Tavares Angelo Cintra
• Grantee: Luciano Tavares Angelo Cintra
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The aim of this study was to evaluate the influence of pulpal infection alone or associated with diabetes in the blood profile and, quantification of proinflammatory cytokines TNF-±, IL-6, IL-17. Will be used 40 Wistar rats divided into 4 groups of 10 animals each: G1 - normal rats, G2 - rats with pulpal infection, G3 - diabetic rats; G4 - diabetic rats with pulpal infection. Diabetes is induced by application of streptozotocin intravenously and confirmed in 24/48 hours by glycemic control. The pulpal infection is induced by exposure of the pulp upper right first molar to the oral environment. After 30 days, the animals' blood will be collected via cardiac puncture to perform the analyzes to characterize the blood profile (CBC), and the measurement of serum proinflammatory cytokines (TNF-±, IL-6, IL-17). Then the animals are sacrificed, the jaws (right and left) and organs (heart, liver and kidney) removed and separated. The maxillary laboratory will be processed for evaluation by light microscopy to characterize the profile of the local disease in each animal. The organs will be analyzed by ELISA for the quantification of proinflammatory cytokines (IL-6, IL-17 and TNF-±). The results obtained in different analyzes will be analyzed and compared with the histological profile, allowing a paired statistical analysis (p <0.05). (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HOLLAND, ROBERTO; GOMES FILHO, JOAO EDUARDO; ANGELO CINTRA, LUCIANO TAVARES; DE AZEVEDO QUEIROZ, INDIA OLINTA; ESTRELA, CARLOS. Factors affecting the periapical healing process of endodontically treated teeth. Journal of Applied Oral Science, v. 25, n. 5, p. 465-476, SEP-OCT 2017. (12/09987-0, 12/02083-8, 12/01153-2, 12/06785-7, 12/06943-1)

ANGELO CINTRA, LUCIANO TAVARES; SAMUEL, RENATA OLIVEIRA; AZUMA, MARIANE MAFFEI; RIBEIRO, CLICIA PEREIRA; NARCISO, LUIS GUSTAVO; FELIX DE LIMA, VALERIA MARCAL; SUMIDA, DORIS HISSAKO; COCLETE, GILBERTO APARECIDO; DEZAN-JUNIOR, ELOI; GOMES-FILHO, JOAO EDUARDO. Apical periodontitis and periodontal disease increase serum IL-17 levels in normoglycemic and diabetic rats. CLINICAL ORAL INVESTIGATIONS, v. 18, n. 9, p. 2123-2128, DEC 2014. (12/02083-8, 11/11337-0)

AZUMA, M. M.; SAMUEL, R. O.; GOMES-FILHO, J. E.; DEZAN-JUNIOR, E.; CINTRA, L. T. A.. The role of IL-6 on apical periodontitis: a systematic review. International Endodontic Journal, v. 47, n. 7, p. 615-621, JUL 2014. (12/02083-8)

ANGELO CINTRA, LUCIANO TAVARES; DA SILVA FACUNDO, AGUINALDO CANDIDO; CARRARA PRIETO, ANNELISE KATRINE; SUMIDA, DORIS HISSAKO; NARCISO, LUIS GUSTAVO; MOGAMI BOMFIM, SUELY REGINA; OLIVEIRA E SILVA, CLEVERSON; DEZAN-JUNIOR, ELOI; GOMES-FILHO, JOAO EDUARDO. Blood Profile and Histology in Oral Infections Associated with Diabetes. JOURNAL OF ENDODONTICS, v. 40, n. 8, p. 1139-1144, AUG 2014. (12/02083-8, 12/04588-0, 10/16999-9)

ANGELO CINTRA, LUCIANO TAVARES; SAMUEL, RENATA OLIVEIRA; CARRARA PRIETO, ANNELISE KATRINE; SUMIDA, DORIS HISSAKO; DEZAN-JUNIOR, ELOI; GOMES-FILHO, JOAO EDUARDO. Oral health, diabetes, and body weight. ARCHIVES OF ORAL BIOLOGY, v. 73, p. 94-99, JAN 2017. (12/02083-8)

CINTRA, L. T. A.; SAMUEL, R. O.; FACUNDO, A. C. S.; PRIETO, A. K. C.; SUMIDA, D. H.; BOMFIM, S. R. M.; SOUZA, J. C.; DEZAN-JUNIOR, E.; GOMES-FILHO, J. E.. Relationships between oral infections and blood glucose concentrations or HbA1c levels in normal and diabetic rats. International Endodontic Journal, v. 47, n. 3, p. 228-237, MAR 2014. (12/02083-8, 10/16999-9)

HOLLAND, ROBERTO; GOMES FILHO, JOAO EDUARDO; ANGELO CINTRA, LUCIANO TAVARES; DE AZEVEDO QUEIROZ, INDIA OLINTA; ESTRELA, CARLOS. Factors affecting the periapical healing process of endodontically treated teeth. Journal of Applied Oral Science, v. 25, n. 5, p. 12-pg., 2017-09-01. (12/09987-0, 12/06785-7, 12/01153-2, 12/06943-1, 12/02083-8)