Influence of diferente irrigating solutions associated to photodinamic therapy: in vitro and in vivo study

• Grant number: 12/06785-7
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2012
• End date: October 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: João Eduardo Gomes Filho
• Grantee: João Eduardo Gomes Filho
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated researchers: Juliane Maria Guerreiro Tanomaru ; Sandra Helena Penha de Oliveira

Abstract

Recently, it has been showed strong evidence of the photodynamic therapy (PDT) success in microbial reduction. PDT is based on appropriate physical, chemical and biological effects, that occur after administration of a photosensitizing agent (PS) activated by visible light of specific wavelength, with intent to destroy the target cell. The aim of this study is to evaluate the behavior of PDT in comparison with some irrigating solutions, as well as the association between the irrigating solutions and PDT using different methodologies: 1-antimicrobial effects in vitro by direct contact test against strains of Enterococcus faecalis and Porphyromonas gingivalis. The results will be evaluated with ANOVA, Tukey and Bonferroni's tests (p <0.05), 2-cytotoxicity in L-929 fibroblasts by the MTT colorimetric method, 3-Production of cytokines (IL-1² and IL-6) by fibroblasts using enzyme immunoassay (ELISA). The results will be statistically analyzed by ANOVA with Bonferroni's correction (p <0.05), 4- tissue response to polyethylene tubes implant in the subcutaneous tissue of rats. The results will be statistically analyzed by Kruskal-Wallis test (p <0.05), 5-Production of cytokines (IL-1² and IL-6) by the tissue, analyzed by ELISA. The results will be statistically analyzed by ANOVA with Bonferroni's correction (p <0.05). (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HOLLAND, ROBERTO; GOMES FILHO, JOAO EDUARDO; ANGELO CINTRA, LUCIANO TAVARES; DE AZEVEDO QUEIROZ, INDIA OLINTA; ESTRELA, CARLOS. Factors affecting the periapical healing process of endodontically treated teeth. Journal of Applied Oral Science, v. 25, n. 5, p. 465-476, SEP-OCT 2017. (12/09987-0, 12/02083-8, 12/01153-2, 12/06785-7, 12/06943-1)

SIVIERI-ARAUJO, GUSTAVO; DE AZEVEDO QUEIROZ, INDIA OLINTA; DAL FABBRO, RENAN; ESTEVES, FERNANDA; ANGELO CINTRA, LUCIANO TAVARES; TOBIAS DUARTE, PAULO CARVALHO; BAGNATO, VANDERLEI SALVADOR; PENHA OLIVEIRA, SANDRA HELENA; GOMES-FILHO, JOAO EDUARDO. Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy. Photodiagnosis and Photodynamic Therapy, v. 18, p. 315-318, JUN 2017. (12/12696-7, 11/23287-8, 12/06785-7)

GOMES-FILHO, JOAO EDUARDO; SIVIERI-ARAUJO, GUSTAVO; SIPERT, CARLA RENATA; DA SILVA SANTOS, LUDMILLA MOTA; DE AZEVEDO QUEIROZ, INDIA OLINTA; MARTINS, CHRISTINE MEN; DO CARMO MAIA, NAYARA KIVILLA; ANGELO CINTRA, LUCIANO TAVARES; DEZAN-JUNIOR, ELOI; BAGNATO, VANDERLEI SALVADOR; et al. Evaluation of photodynamic therapy on fibroblast viability and cytokine production. Photodiagnosis and Photodynamic Therapy, v. 13, p. 97-100, MAR 2016. (12/06785-7, 11/23287-8, 12/12695-0)

HOLLAND, ROBERTO; GOMES FILHO, JOAO EDUARDO; ANGELO CINTRA, LUCIANO TAVARES; DE AZEVEDO QUEIROZ, INDIA OLINTA; ESTRELA, CARLOS. Factors affecting the periapical healing process of endodontically treated teeth. Journal of Applied Oral Science, v. 25, n. 5, p. 12-pg., 2017-09-01. (12/09987-0, 12/06785-7, 12/01153-2, 12/06943-1, 12/02083-8)