Metabolomics for non-invasive evaluation of in vitro produced bovine embryos

• Grant number: 12/10351-2
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2012
• End date: April 30, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marcella Pecora Milazzotto
• Grantee: Marcella Pecora Milazzotto
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated researchers: Herculano da Silva Martinho

Abstract

Currently, several technologies have been explored and have been suggested to evidence metabolic differences between embryos to identifying those most able to generate pregnancy. However, the commercial application of these technologies remains a challenge, mainly because they require very complex equipment and skilled labor, which seems to be financially unfeasible for commercial application. Thus, there remains a need to develop a technology to predict the viability of embryos in a non-invasive, quick and economically feasible to its comercial application. In this way, vibrational spectroscopy has been studied by allowing the rapid and nondestructive analysis of various types of biological samples, including culture media. This technology is based on the evaluation of the interaction of a sample with electromagnetic radiation. The combination of vibrational spectroscopy with biochemical, morphological and genomics evaluation will allow the more sensitive identification of higher quality embryos. The objective of this project is to determine biochemical, morphological and molecular biomarkers that may be related to embryo viability. In addition, the identification of a spectroscopic pattern characteristic of these molecules in embryo culture medium, allowing the non-invasive characterization of embryo quality and viability, contributing to the improvement of in vitro production of bovine embryos. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, ERIKA CRISTINA; MARTINHO, HERCULANO; ANNES, KELLY; DA SILVA, THAIS; SOARES, CARLOS ALEXANDRE; LEITE, ROBERTA FERREIRA; MILAZZOTTO, MARCELLA PECORA. Raman-based noninvasive metabolic profile evaluation of in vitro bovine embryos. Journal of Biomedical Optics, v. 21, n. 7, JUL 2016. (12/10351-2)

DOS SANTOS, ERIKA CRISTINA; DE LIMA, CAMILA BRUNA; ANNES, KELLY; MILAZZOTTO, MARCELLA PECORA. Noninvasive characterization of metabolites secreted in culture media by bovine embryos during in vitro production. METABOLOMICS, v. 12, n. 5, MAY 2016. (12/10351-2)

MILAZZOTTO, MARCELLA PECORA; GOISSIS, MARCELO DEMARCHI; CHITWOOD, JAMES LEE; ANNES, KELLY; SOARES, CARLOS ALEXANDRE; ISPADA, JESSICA; ORTIZ AVILA ASSUMPCAO, MAYRA ELENA; ROSS, PABLO JUAN. Early cleavages influence the molecular and the metabolic pattern of individually cultured bovine blastocysts. Molecular Reproduction and Development, v. 83, n. 4, p. 324-336, APR 2016. (13/13199-0, 12/20535-3, 12/10351-2)

ANNES, KELLY; SUDANO, MATEUS JOSE; BELAZ, KATIA ROBERTA A.; TATA, ALESSANDRA; SANTOS, VANESSA GONCALVES; DA FONSECA JUNIOR, ALDCEJAM MARTINS; DOS SANTOS, ERIKA CRISTINA; EBERLIN, MARCOS NOGUEIRA; MILAZZOTTO, MARCELLA PECORA. Lipid characterization of in vitro-produced bovine embryos with distinct kinetics of development. ZYGOTE, v. 27, n. 6, p. 413-422, DEC 2019. (11/06191-7, 12/10351-2, 12/07206-0, 13/13199-0)

ALVES, GLAUCIA PEREIRA; BERTUCCEZ CORDEIRO, FERNANDA; DE LIMA, CAMILA BRUNA; ANNES, KELLY; DOS SANTOS, ERIKA CRISTINA; ISPADA, JESSICA; FONTES, PATRICIA KUBO; GOUVEIA NOGUEIRA, MARCELO FABIO; NICHI, MARCILIO; MILAZZOTTO, MARCELLA PECORA. Follicular environment as a predictive tool for embryo development and kinetics in cattle. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 31, n. 3, p. 451-461, FEB 2019. (12/10351-2, 12/50533-2, 15/03381-0)

SILVA, T.; SANTOS, E. C.; ANNES, K.; SOARES, C. A.; LEITE, R. F.; LIMA, C. B.; MILAZZOTTO, M. P.. Morphokinetic-related response to stress in individually cultured bovine embryos. Theriogenology, v. 86, n. 5, p. 1308-1317, SEP 15 2016. (12/10351-2)

Filed patent(s) as a result of this research project