Assessment of effects of propolis and its bioactive compounds on the modulation of of macrophages inflammatory response

• Grant number: 12/14323-3
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2012
• End date: March 31, 2015
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Marcia Pinto Alves Mayer
• Grantee: Marcia Pinto Alves Mayer
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Ellen Sayuri Ando Suguimoto ; Pedro Luiz Rosalen ; Severino Matias de Alencar

Abstract

The Brazilian propolis stands out among the natural products because it is considered a rich source for discovering new bioactive compounds. Among Brazilian propolis, the red one (type 13) exhibited antimicrobial, antitumor and antioxidant activities. Moreover, the main chemical constituent is the isoflavones, that distinct it from any other type of Brazilian propolis. Recently, neovestitol and vestitol were isolated and identified. These compounds showed antimicrobial, anticaries and antioxidant activities. However, there are no reports in the literature about not only to anti-inflammatory properties of propolis, but also about modulation of macrophage activity. However, previous studies in our laboratory have revealed such activities. Hypothesis: propolis and its bioactive compounds have anti-inflammatory properties through modulation of macrophages' activity. Purpose: This project aims to evaluate the effects of propolis and its active compounds on the activation of RAW 264.7 and peritoneal macrophages as well as determine its mechanism of action. Methods: The methods used to achieve the proposed objectives are: 1) Collection and monitored fractionation of propolis aiming to isolation of bioactive compounds, b) analysis of nitric oxide (NO) and cytokines production by macrophages, b) analysis of genes transcription associated with signal transductions pathways, cytokine and NO production, c) analysis of receptors profile, expressed by activated macrophages (CD80/86 - classical activation and MCR1- alternative activation). (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BUENO-SILVA, BRUNO; KAWAMOTO, DIONE; ANDO-SUGUIMOTO, ELLEN S.; ALENCAR, SEVERINO M.; ROSALEN, PEDRO L.; MAYER, MARCIA P. A.. Brazilian Red Propolis Attenuates Inflammatory Signaling Cascade in LPS-Activated Macrophages. PLoS One, v. 10, n. 12, DEC 14 2015. (12/14323-3, 12/01500-4)

BUENO-SILVA, BRUNO; KAWAMOTO, DIONE; ANDO-SUGUIMOTO, ELLEN S.; CASARIN, RENATO C. V.; ALENCAR, SEVERINO M.; ROSALEN, PEDRO L.; MAYER, MARCIA P. A.. Brazilian red propolis effects on peritoneal macrophage activity: Nitric oxide, cell viability, pro-inflammatory cytokines and gene expression. Journal of Ethnopharmacology, v. 207, p. 100-107, JUL 31 2017. (12/14323-3, 12/01500-4)

BUENO-SILVA, BRUNO; ROSALEN, PEDRO L.; ALENCAR, SEVERINO M.; MAYER, MARCIA P. A.. Anti-inflammatory mechanisms of neovestitol from Brazilian red propolis in LPS-activated macrophages. Journal of Functional Foods, v. 36, p. 440-447, SEP 2017. (12/14323-3, 12/01500-4)

BUENO-SILVA, BRUNO; BUENO, MANUELA ROCHA; KAWAMOTO, DIONE; CASARIN, RENATO C.; SPESSOTO PINGUEIRO, JOAO MARCOS; ALENCAR, SEVERINO MATIAS; ROSALEN, PEDRO LUIZ; ALVES MAYER, MARCIA PINTO. Anti-Inflammatory Effects of (3S)-Vestitol on Peritoneal Macrophages. PHARMACEUTICALS, v. 15, n. 5, p. 10-pg., 2022-05-01. (12/01500-4, 12/14323-3)

BUENO-SILVA, BRUNO; ROSALEN, PEDRO L.; ALENCAR, SEVERINO M.; MAYER, MARCIA P. A.. Vestitol drives LPS-activated macrophages into M2 phenotype through modulation of NF-kappa B pathway. International Immunopharmacology, v. 82, MAY 2020. (12/14323-3, 12/01500-4)

Filed patent(s) as a result of this research project