| Grant number: |
12/01500-4
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| Support type: | Scholarships in Brazil - Post-Doctorate |
| Effective date (Start): |
June 01, 2012
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| Effective date (End): |
May 31, 2015
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| Field of knowledge: | Health Sciences
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Dentistry
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Social and Preventive Dentistry |
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| Principal Investigator: | Marcia Pinto Alves Mayer |
| Grantee: | Bruno Bueno Silva |
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| Home Institution: |
Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
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Abstract
The Brazilian propolis stands out among the natural products because it is considered a rich source for discovering new bioactive compounds. Among Brazilian propolis, the red one (type 13) exhibited antimicrobial, antitumor and antioxidant activities. Moreover, the main chemical constituent is the isoflavones, that distinct it from any other type of Brazilian propolis. Recently, neovestitol and vestitol were isolated and identified. These compounds showed antimicrobial, anticaries and antioxidant activities. However, there are no reports in the literature about not only to anti-inflammatory properties of propolis, but also about modulation of macrophage activity. However, previous studies in our laboratory have revealed such activities. Hypothesis: propolis and its bioactive compounds have anti-inflammatory properties through modulation of macrophages' activity. Purpose: This project aims to evaluate the effects of propolis and its active compounds on the activation of RAW 264.7 and peritoneal macrophages as well as determine its mechanism of action. Methods: The methods used to achieve the proposed objectives are: 1) Collection and monitored fractionation of propolis aiming to isolation of bioactive compounds, b) analysis of nitric oxide (NO) and cytokines production by macrophages, b) analysis of genes transcription associated with signal transductions pathways, cytokine and NO production, c) analysis of receptors profile, expressed by activated macrophages (CD80/86 - classical activation and MCR1- alternative activation).
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Scientific publications
(6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BUENO-SILVA, BRUNO;
KAWAMOTO, DIONE;
ANDO-SUGUIMOTO, ELLEN S.;
CASARIN, RENATO C. V.;
ALENCAR, SEVERINO M.;
ROSALEN, PEDRO L.;
MAYER, MARCIA P. A.
Brazilian red propolis effects on peritoneal macrophage activity: Nitric oxide, cell viability, pro-inflammatory cytokines and gene expression.
Journal of Ethnopharmacology,
v. 207,
p. 100-107,
JUL 31 2017.
Web of Science Citations: 11.
BUENO-SILVA, BRUNO;
FRANCHIN, MARCELO;
ALVES, CLAUDINEY DE FREITAS;
DENNY, CARINA;
COLON, DAVID FERNANDO;
CUNHA, THIAGO MATTAR;
ALENCAR, SEVERINO MATIAS;
NAPIMOGA, MARCELO HENRIQUE;
ROSALEN, PEDRO LUIZ.
Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process.
Phytomedicine,
v. 23,
n. 13,
p. 1583-1590,
DEC 1 2016.
Web of Science Citations: 15.