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Assessment of effects of propolis and its bioactive compounds on the modulation of of macrophages inflammatory response

Grant number: 12/01500-4
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): June 01, 2012
Effective date (End): May 31, 2015
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal Investigator:Marcia Pinto Alves Mayer
Grantee:Bruno Bueno Silva
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The Brazilian propolis stands out among the natural products because it is considered a rich source for discovering new bioactive compounds. Among Brazilian propolis, the red one (type 13) exhibited antimicrobial, antitumor and antioxidant activities. Moreover, the main chemical constituent is the isoflavones, that distinct it from any other type of Brazilian propolis. Recently, neovestitol and vestitol were isolated and identified. These compounds showed antimicrobial, anticaries and antioxidant activities. However, there are no reports in the literature about not only to anti-inflammatory properties of propolis, but also about modulation of macrophage activity. However, previous studies in our laboratory have revealed such activities. Hypothesis: propolis and its bioactive compounds have anti-inflammatory properties through modulation of macrophages' activity. Purpose: This project aims to evaluate the effects of propolis and its active compounds on the activation of RAW 264.7 and peritoneal macrophages as well as determine its mechanism of action. Methods: The methods used to achieve the proposed objectives are: 1) Collection and monitored fractionation of propolis aiming to isolation of bioactive compounds, b) analysis of nitric oxide (NO) and cytokines production by macrophages, b) analysis of genes transcription associated with signal transductions pathways, cytokine and NO production, c) analysis of receptors profile, expressed by activated macrophages (CD80/86 - classical activation and MCR1- alternative activation).

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BUENO-SILVA, BRUNO; ROSALEN, PEDRO L.; ALENCAR, SEVERINO M.; MAYER, MARCIA P. A. Vestitol drives LPS-activated macrophages into M2 phenotype through modulation of NF-kappa B pathway. International Immunopharmacology, v. 82, MAY 2020. Web of Science Citations: 0.
BUENO-SILVA, BRUNO; ROSALEN, PEDRO L.; ALENCAR, SEVERINO M.; MAYER, MARCIA P. A. Anti-inflammatory mechanisms of neovestitol from Brazilian red propolis in LPS-activated macrophages. Journal of Functional Foods, v. 36, p. 440-447, SEP 2017. Web of Science Citations: 6.
BUENO-SILVA, BRUNO; KAWAMOTO, DIONE; ANDO-SUGUIMOTO, ELLEN S.; CASARIN, RENATO C. V.; ALENCAR, SEVERINO M.; ROSALEN, PEDRO L.; MAYER, MARCIA P. A. Brazilian red propolis effects on peritoneal macrophage activity: Nitric oxide, cell viability, pro-inflammatory cytokines and gene expression. Journal of Ethnopharmacology, v. 207, p. 100-107, JUL 31 2017. Web of Science Citations: 11.
BUENO-SILVA, BRUNO; FRANCHIN, MARCELO; ALVES, CLAUDINEY DE FREITAS; DENNY, CARINA; COLON, DAVID FERNANDO; CUNHA, THIAGO MATTAR; ALENCAR, SEVERINO MATIAS; NAPIMOGA, MARCELO HENRIQUE; ROSALEN, PEDRO LUIZ. Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process. Phytomedicine, v. 23, n. 13, p. 1583-1590, DEC 1 2016. Web of Science Citations: 15.
BUENO-SILVA, BRUNO; KAWAMOTO, DIONE; ANDO-SUGUIMOTO, ELLEN S.; ALENCAR, SEVERINO M.; ROSALEN, PEDRO L.; MAYER, MARCIA P. A. Brazilian Red Propolis Attenuates Inflammatory Signaling Cascade in LPS-Activated Macrophages. PLoS One, v. 10, n. 12 DEC 14 2015. Web of Science Citations: 17.
FREIRES, IRLAN ALMEIDA; BUENO-SILVA, BRUNO; DE CARVALHO GALVAO, LIVIA CAMARA; TEIXEIRA DUARTE, MARTA CRISTINA; SARTORATTO, ADILSON; FIGUEIRA, GLYN MARA; DE ALENCAR, SEVERINO MATIAS; ROSALEN, PEDRO LUIZ. The Effect of Essential Oils and Bioactive Fractions on Streptococcus mutans and Candida albicans Biofilms: A Confocal Analysis. Evidence-based Complementary and Alternative Medicine, 2015. Web of Science Citations: 6.

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Filed patent(s) as a result of this research project

USO DA COMBINAÇÃO NEOVESTITOL E VESTITOL PARA TRATAMENTO E PREVENÇÃO DA ATEROSCLEROSE BR1020170166031 - Universidade Estadual de Campinas (UNICAMP) . Pedro Luiz Rosalen; Severino M. Alencar; Marcia Pinto Alves Mayer; Bruno Bueno Silva - August 2017, 02