Role of peptides p217 and p290 on activation of ins-r in salivary glands of diabetic mice

• Grant number: 12/18012-2
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2012
• End date: May 31, 2015
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Eduardo José Caldeira
• Grantee: Eduardo José Caldeira
• Host Institution: Faculdade de Medicina de Jundiaí (FMJ). Prefeitura Municipal de Jundiaí. Jundiaí , SP, Brazil

Abstract

Diabetes mellitus is a disease that affect different tissues and organs, including the salivary glands which have similar biological structure, in some respects, to pancreas. Current studies have used immunomodulators in an attempt to reverse the tissue damage caused by the hyperglycaemic condition. However, the toxic effects and risks of immune suppression are a limitation to use of these drugs. Thus, is necessary the continuous search for other therapies, with less risk and more effectives to control the diabetes and especially to the reversal of lesions associated to this condition. Then, the objective of this study is to evaluate the effects of treatment with peptides P217 and p290 of glutamic acid decarboxylase (GAD) on the cells responsible by production of saliva in parotid and submandibular glands of diabetic mice, observing mainly the therapeutic effect on recovery of interaction between insulin and receptors in these tissues. A total of 21 mice will be divided into 3 groups: Group I, with 7 Balb / C (controls mice), Group II, with 7 NOD (diabetic mice) and the Group III with 7 NOD (diabetic mice) that will receive the peptide P217 and P290 of glutamic acid decarboxylase. During treatment in all animals will analysed the solid and liquid intake and glucose levels. After the experimental period, samples of salivary tissues will be processed to morphological analysis, and also immunofluorescence and immunoblotting for insulin receptors (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS, GEOVANE RIBEIRO; CUNHA, MARCELO RODRIGUES; CALDEIRA, EDUARDO JOSE; GALDEANO, EWERTON ALEXANDRE; SEABRA PRUDENTE, RAPHAEL CRUZ; LOPES PINTO, CLOVIS ANTONIO. Effect of antioxidant treatment with n-acetylcysteine and swimming on lipid expression of sebaceous glands in diabetic mice. SCIENTIFIC REPORTS, v. 11, n. 1, JUN 7 2021. (12/18012-2)

DA SILVA, RODRIGO EDUARDO; CERVIGNE, NILVA DE KARLA; PINTO, CLOVIS ANTONIO LOPES; DA CUNHA, MARCELO RODRIGUES; ROJAS, FERNANDA; PERONI, LUIS ANTONIO; AUGUSTO, TAIZE MACHADO; MARCHI, EVALDO; KHAN, BARKAT ALI; CALDEIRA, EDUARDO JOSE. Role of GAD peptides p217 and p290 in the repair of INS receptor in salivary tissues of type 1 diabetic mice. Pakistan Journal of Pharmaceutical Sciences, v. 30, n. 5, p. 1545-1550, SEP 2017. (12/18012-2)