Influence of lidocaine, bupivacaine and ropivacaine on the secretion of neuropeptides CGRP and SP in rats skin

• Grant number: 12/19163-4
• Support Opportunities: Regular Research Grants
• Start date: January 01, 2013
• End date: December 31, 2014
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Lydia Masako Ferreira
• Grantee: Lydia Masako Ferreira
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Bernardo Sergio Hochman Rzeszetkowski ; Guilherme Abbud Franco Lapin

Abstract

Local anesthetics (LA) has widespread use in recent times due to its effectiveness and safety. The neurogenic inflammation constitutes the release of proinflammatory cutaneous neuropeptides in consequence of a nociceptive stimulus running along the axon. These substances, liberated by the nerve endings of the dermis by a cascading local effect, help to quickly start the inflammatory phase of healing. However, studies show that LA can slow the early stages of wound healing by blocking nerve cell and the release of neuropeptides and, consequently, the early trigger of the inflammatory phase. Objective: To evaluate the effect of the local anesthetic lidocaine (LD), bupivacaine (BP) and ropivacaine (PR) in the secretion of neuropeptides CGRP and SP in rat skin. Methods: To test the AL in three experimental groups with LD, BP and PR and a control group with saline 0.9%, consisting of seven rats each. After general anesthesia, the drug will be injected in each group along a straight line on the dorsum of each rat and an incision is made (nociception stimulus) along this line. The mouse is sacrificed soon after, and the skin of the animal's back, containing the incised line will be removed with an electric dermatome. From the medium point of the line, it will be taken a sample of skin with an 8mm punch. This sample is sent for analysis by Western blot for quantification of CGRP and SP blindly for each drug group. The results are for statistical analysis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LAPIN, GUILHERME A. F.; HOCHMAN, BERNARDO; MAXIMINO, JESSICA R.; CHADI, GERSON; FERREIRA, LYDIA M.. Effects of Lidocaine, Bupivacaine, and Ropivacaine on Calcitonin Gene-Related Peptide and Substance P Levels in the Incised Rat Skin. ADVANCES IN SKIN & WOUND CARE, v. 29, n. 4, p. 169-177, APR 2016. (12/19163-4)