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Modulatory effects of local anesthetics associated with carriers on the production of inflammatory mediators and cell viability in oral epithelial cells and human gingival fibroblasts

Grant number: 12/07310-2
Support type:Regular Research Grants
Duration: July 01, 2012 - June 30, 2014
Field of knowledge:Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
Principal Investigator:Francisco Carlos Groppo
Grantee:Francisco Carlos Groppo
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Assoc. researchers:Francisco Carlos Groppo


The most effective pain control has motivated the research for new local anesthetic formulations. The association with lipossomal or ciclodextrin carriers has been proposed in order to prolong the anesthetic effect and reducing its toxicity. New anesthetic formulations produced a low systemic toxicity and longer duration of anesthesia. Adverse effects were also observed. One of the side effects was the appearance of inflammatory painless process without apparent cause. This inflammatory response of unknown nature could be triggered by the release of proinflammatory mediators. This study aims to check the effects of local anesthetics and associated with carriers on the modulation of inflammatory mediators IL-6, IL1-±, IL-8, TNF-±, IL-10 and PGE2, in addition to its action on cell viability and Caspase-3 production in cultured oral epithelial cells and human gingival fibroblasts. The cells will be exposed to different concentrations of anesthetics bupivacaine, lidocaine, ropivacaine, and benzocaine free or associated with liposomes or cyclodextrin. Quantification of cytokines and PGE2 will be done using the capture ELISA after 6h and 24h. Cell viability will be estimated after the exposure period and compared to the untreated control group. Statistical analysis will be performed by ANOVA (Tukey test) or Kruskal-Wallis test (Dunn), BioEstat 5.0 statistical package (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NUNES FERREIRA, LUIZ EDUARDO; MUNIZ, BRUNO VILELA; BURGA-SANCHEZ, JONNY; VOLPATO, MARIA CRISTINA; DE PAULA, ENEIDA; RIBEIRO ROSA, EDVALDO ANTONIO; GROPPO, FRANCISCO CARLOS. The effect of two drug delivery systems in ropivacaine cytotoxicity and cytokine release by human keratinocytes and fibroblasts. Journal of Pharmacy and Pharmacology, v. 69, n. 2, p. 161-171, FEB 2017. Web of Science Citations: 5.
NUNES FERREIRA, LUIZ EDUARDO; MUNIZ, BRUNO VILELA; DOS SANTOS, CLEITON PITA; VOLPATO, MARIA CRISTINA; DE PAULA, ENEIDA; GROPPO, FRANCISCO CARLOS. Comparison of liposomal and 2-hydroxypropyl-beta-cyclodextrin-lidocaine on cell viability and inflammatory response in human keratinocytes and gingival fibroblasts. Journal of Pharmacy and Pharmacology, v. 68, n. 6, p. 791-802, JUN 2016. Web of Science Citations: 2.

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