Modulatory effects of local anesthetics associated with carriers on the production of inflammatory mediators and cell viability in oral epithelial cells and human gingival fibroblasts

• Grant number: 12/07310-2
• Support Opportunities: Regular Research Grants
• Duration: July 01, 2012 - June 30, 2014
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Principal Investigator: Francisco Carlos Groppo
• Grantee: Francisco Carlos Groppo
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated researchers: Francisco Carlos Groppo

Abstract

The most effective pain control has motivated the research for new local anesthetic formulations. The association with lipossomal or ciclodextrin carriers has been proposed in order to prolong the anesthetic effect and reducing its toxicity. New anesthetic formulations produced a low systemic toxicity and longer duration of anesthesia. Adverse effects were also observed. One of the side effects was the appearance of inflammatory painless process without apparent cause. This inflammatory response of unknown nature could be triggered by the release of proinflammatory mediators. This study aims to check the effects of local anesthetics and associated with carriers on the modulation of inflammatory mediators IL-6, IL1-±, IL-8, TNF-±, IL-10 and PGE2, in addition to its action on cell viability and Caspase-3 production in cultured oral epithelial cells and human gingival fibroblasts. The cells will be exposed to different concentrations of anesthetics bupivacaine, lidocaine, ropivacaine, and benzocaine free or associated with liposomes or cyclodextrin. Quantification of cytokines and PGE2 will be done using the capture ELISA after 6h and 24h. Cell viability will be estimated after the exposure period and compared to the untreated control group. Statistical analysis will be performed by ANOVA (Tukey test) or Kruskal-Wallis test (Dunn), BioEstat 5.0 statistical package (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, CAMILA BATISTA; VOLPATO, MARIA CRISTINA; MUNIZ, BRUNO VILELA; DOS SANTOS, CLEITON PITA; SERPE, LUCIANO; NUNES FERREIRA, LUIZ EDUARDO; SILVA DE MELO, NATHALIE FERREIRA; FRACETO, LEONARDO FERNANDES; GROPPO, FRANCISCO CARLOS; FRANZ-MONTAN, MICHELLE. Promising potential of articaine-loaded poly(epsilon-caprolactone) nanocapules for intraoral topical anesthesia. PLoS One, v. 16, n. 2, FEB 11 2021. (12/06974-4, 12/02539-1, 12/07310-2)

NUNES FERREIRA, LUIZ EDUARDO; MUNIZ, BRUNO VILELA; DOS SANTOS, CLEITON PITA; VOLPATO, MARIA CRISTINA; DE PAULA, ENEIDA; GROPPO, FRANCISCO CARLOS. Comparison of liposomal and 2-hydroxypropyl-beta-cyclodextrin-lidocaine on cell viability and inflammatory response in human keratinocytes and gingival fibroblasts. Journal of Pharmacy and Pharmacology, v. 68, n. 6, p. 791-802, JUN 2016. (12/07310-2, 11/12666-8)

NUNES FERREIRA, LUIZ EDUARDO; MUNIZ, BRUNO VILELA; BURGA-SANCHEZ, JONNY; VOLPATO, MARIA CRISTINA; DE PAULA, ENEIDA; RIBEIRO ROSA, EDVALDO ANTONIO; GROPPO, FRANCISCO CARLOS. The effect of two drug delivery systems in ropivacaine cytotoxicity and cytokine release by human keratinocytes and fibroblasts. Journal of Pharmacy and Pharmacology, v. 69, n. 2, p. 161-171, FEB 2017. (12/07310-2, 11/12666-8)