| Full text | |
| Author(s): |
da Silva, Camila Batista
[1, 2]
;
Volpato, Maria Cristina
[1]
;
Muniz, Bruno Vilela
[1, 3]
;
dos Santos, Cleiton Pita
[1]
;
Serpe, Luciano
[4, 1]
;
Nunes Ferreira, Luiz Eduardo
[1, 5]
;
Silva de Melo, Nathalie Ferreira
[6, 7]
;
Fraceto, Leonardo Fernandes
[6]
;
Groppo, Francisco Carlos
[1]
;
Franz-Montan, Michelle
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Biosci, Piracicaba, SP - Brazil
[2] Univ Mogi das Cruzes UMC, Hlth Sci, Mogi Das Cruzes, SP - Brazil
[3] Itapeva Fac Social & Agr Sci FAIT, Itapeva, SP - Brazil
[4] Univ Estadual Ponta Grossa, Dept Dent, Ponta Grossa, Parana - Brazil
[5] Guarulhos Univ UNG, Lab Inflammat & Immunol, Guarulhos, SP - Brazil
[6] Sao Paulo State Univ, Dept Environm Engn, Sorocaba, SP - Brazil
[7] Sao Leopoldo Mandic Res Inst, Dept Immunol & Mol Biol, Campinas, SP - Brazil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | PLoS One; v. 16, n. 2 FEB 11 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
To determine whether the permeation capacity and analgesic efficacy of articaine (ATC) could be increased and cytotoxicity decreased by encapsulation in poly(e-caprolactone) nanocapsules (ATC(nano)), aiming at local or topical anesthesia in dentistry. Cellular viability was evaluated (using the MTT test and fluorescence microscopy) after 1 h and 24 h exposure of HaCaT cells to ATC, ATC(nano), ATC with epinephrine (ATC(epi)), and ATC in nanocapsules with epinephrine (ATC(nanoepi)). The profiles of permeation of 2% ATC and 2% ATC(nano) across swine esophageal epithelium were determined using Franz-type vertical diffusion cells. Analgesic efficacy was evaluated with a von Frey anesthesiometer in a postoperative pain model in rats, comparing the 2% ATC, 2% ATC(nano), 2% ATC(epi), and 2% ATC(nanoepi) formulations to 4% ATC(epi) (a commercially available formulation). We show that use of the nanocapsules decreased the toxicity of articaine (P<0.0001) and increased its flux (P = 0.0007). The 2% ATC(epi) and 4% ATC(epi) formulations provided higher analgesia success and duration (P<0.05), compared to 2% ATC, 2% ATC(nano), and 2% ATC(nanoepi). Articaine-loaded poly(e-caprolactone) nanocapsules constitute a promising formulation for intraoral topical anesthesia (prior to local anesthetic injection), although it is not effective when injected in inflamed tissues for pain control, such as irreversible pulpitis. (AU) | |
| FAPESP's process: | 12/06974-4 - Development, biocompatibility and permeation studies on gel formulations of poly-epsilon-caprolactone nanocapsules containing local anesthetics |
| Grantee: | Michelle Franz Montan Braga Leite |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 12/02539-1 - Biocompatibity and permeation studies of articaine-loaded poly(epsilon-caprolactone) nanocapules formulations. |
| Grantee: | Camila Batista da Silva de Araujo Candido |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 12/07310-2 - Modulatory effects of local anesthetics associated with carriers on the production of inflammatory mediators and cell viability in oral epithelial cells and human gingival fibroblasts |
| Grantee: | Francisco Carlos Groppo |
| Support Opportunities: | Regular Research Grants |