Comparison of liposomal and 2-hydroxypropyl-beta-c... - BV FAPESP
Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparison of liposomal and 2-hydroxypropyl-beta-cyclodextrin-lidocaine on cell viability and inflammatory response in human keratinocytes and gingival fibroblasts

Full text
Author(s):
Nunes Ferreira, Luiz Eduardo [1] ; Muniz, Bruno Vilela [1] ; dos Santos, Cleiton Pita [1] ; Volpato, Maria Cristina [1] ; de Paula, Eneida [1] ; Groppo, Francisco Carlos [1]
Total Authors: 6
Affiliation:
[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Physiol Sci, Limeira Ave 901, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Pharmacy and Pharmacology; v. 68, n. 6, p. 791-802, JUN 2016.
Web of Science Citations: 2
Abstract

Objectives The aim of this study was to observe the effect multilamellar liposomes (MLV) and 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) in the in-vitro effects of lidocaine in cell viability, pro-inflammatory cytokines and prostaglandin E-2 release of both human keratinocytes (HaCaT) and gingival fibroblasts (HGF) cells. Methods HaCaT and HGF cells were exposed to lidocaine 100-1 mu M in plain, MLV and HP-beta-CD formulations for 6 h or 24 h. The formulation effects in cell viability were measured by XTT assay and by fluorescent labelling. Cytokines (IL-8, IL-6 and TNF-alpha) and PGE(2) release were quantified by ELISA. Key findings MLV and HP-beta-CD formulations did not affect the HaCaT viability, which was significantly decreased by plain lidocaine after 24 h of exposure. Both drug carriers increased all cytokines released by HGF after 24-h exposure, and none of the carriers was able to reduce the PGE(2) release induced by lidocaine. Conclusion The effect of drug carrier in the lidocaine effects was dependent on the cell type, concentration and time of exposure. MLV and HP-beta-CD showed benefits in improving cell viability; however, both of them showed a tendency to increase cytokine release when compared to the plain solution. (AU)

FAPESP's process: 12/07310-2 - Modulatory effects of local anesthetics associated with carriers on the production of inflammatory mediators and cell viability in oral epithelial cells and human gingival fibroblasts
Grantee:Francisco Carlos Groppo
Support Opportunities: Regular Research Grants
FAPESP's process: 11/12666-8 - Effects of local anesthetic associated with carriers in the modulation of inflammatory mediators, cell viability and apoptosis induction
Grantee:Luiz Eduardo Nunes Ferreira
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)