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Modulatory effects of 2%, 3% and 4% plain articaine and articaine encapsulated into liposomes on citocine production by human gingival fibroblasts

Grant number: 11/15768-6
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): February 28, 2014
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Maria Cristina Volpato
Grantee:Bruno Vilela Muniz
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Associated research grant:06/00121-9 - New formulations for the controlled release of local anesthetics in dentistry: from development to clinical tests, AP.TEM

Abstract

The local anesthetic toxicity has been shown in many cell types and may be associated to the production of proinflammatory cytokines. Articaine has been implicated in many cases of paresthesia after inferior alveolar nerve block; this occurrence seems to be concentration-dependent. The association to carriers (objective of the project "temático" associated), such as liposomes, has been shown to reduce systemic toxicity, to increase the plasmatic half life "in vivo" and to increase anesthesia duration in some models. Therefore, the aim of this study is to analyze the effects of articaine on the modulation of IL6 and IL1± cytokines, on caspase 3 and on the celular viability in human gengival fibroblats. The cells will be exposed to 2%, 3% and 4% plain articaine, 2%, 3% and 4% articaine encapsulated into unilamelar liposomes and controls (saline, liposomal suspension and without treatment). IL6 and IL1± cytokines (Elisa immunoassay), caspase 3 (fluorimetric assay) and cellular viability (XTT reduction - spectrophotometry) will be evaluated at 10min and 4h after exposure to the treatments. Statistical analysis will be performed with BioEstat 5.0. Data will be submitted to ANOVA (Tukey test) or Kruskal-Wallis (Dunn test) depending on normality of distribution and homocedasticity of variances. Significance level will be set at 5%.

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MUNIZ, Bruno Vilela. The effects of plain and liposome-associated articaine with transmembrane pH gradient on humam keratinocytes (HaCaT) viability and IL-6 expression. 2014. Master's Dissertation - Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba.

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