Analysis of the concentration of collagen in wound healing in rats treated with Mitomycin C

• Grant number: 12/22383-6
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2013
• End date: February 28, 2015
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Fernando de Andrade Quintanilha Ribeiro
• Grantee: Fernando de Andrade Quintanilha Ribeiro
• Host Institution: Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP). Fundação Arnaldo Vieira de Carvalho. São Paulo , SP, Brazil

Abstract

Wound healing involves three physiological stages: inflammatory, proliferative and remodeling phases. Failure or lengthening of one of those phases may delay the complete repair or even cause persistence of the open wound. In proliferative phase occurs collagen type III deposition and in remodeling phase this collagen is replaced by collagen type I. Mitomycin-C is a chemotherapy drug with cytotoxic action. This easy administration drug may be used to delay the tissue repair process in surgical wounds, thus avoiding stenosis.Objective: To study the histological aspects of the skin healing process of surgical wounds produced in mice, at the 30th and 60th post-operative days, under the action of mitomycin-C , analyzing total collagen concentration and collagen type I and III concentration. Methods: Round wounds created in the dorsal skin of 35 wistar rats, following the procedures of a secondary intention excision dermal wound model with two groups: 22 animals treated with mitomycin-C and 13 untreated animals (control).In the 30th and 60th post operative days, the scars were surgically removed and submitted to picrosirius red staining, analyzing collagen concentration by optical microscopy and type I and type III by polarized light microscopy.Expected results: At the 30th post operative day, we expect a decrease of total collagen concentration in mitomycin-C group, with higher concentration of collagen type III than type I. At the 60th post operative day, in this same group, we expect an increase of total collagen concentration with higher concentration of collagen type I than type III. (AU)