Relations of immunostainings of cancer stem cells (CD44+/CD24-) with prognostic factors in canine mammary neoplasms

• Grant number: 13/01843-1
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2013
• End date: October 31, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Geórgia Modé Magalhães
• Grantee: Geórgia Modé Magalhães
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil
• Associated researchers: Alfredo Ribeiro da Silva ; Renee Laufer Amorim ; sabryna gouveia calazans

Abstract

The presence of tumor cancer cells (CSCs) may indicate a worse prognosis, since these are more resistant to chemotherapy and relate to distant metastases. The canine mammary tumors are very common and the presence of CSCs compared with prognostic factors may help in the understanding of breast oncogenesis. There will be assessed 110 canine mammary neoplasms divided into solid carcinomas grade II and III, tubular, and papillary carcinomas in mixed tumors grades I, II and III. After defining the phenotype immunohistochemical CD44+/CD24- by correlation analysis of Pearson, the results will be compared with immunostaining for Ki-67, p53, and cadherin, Caspase 3, c-erb2, pan cytokeratin, vimentin, VEGF receptors progesterone and estrogen by ANOVA and Tukey. The immunostainings will be quantified and related to histological type and degree of malignancy of the tumor. (AU)