Comparison of the effect of two protocols of chronic stress in cardiovascular and autonomic functions in rats

• Grant number: 12/14376-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2013
• End date: May 31, 2015
• Field of knowledge: Biological Sciences - Pharmacology - Neuropsychopharmacology
• Principal Investigator: Carlos Cesar Crestani
• Grantee: Carlos Cesar Crestani
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Studies have demonstrated a direct relationship between stress and cardiovascular diseases. Although the importance of this issue, the mechanisms involved in association between stress and cardiovascular complications was poorly investigated.It has been proposed that behavioral and physiological changes evoked by stress are related to characteristics of aversive stimuli (eg., type, duration, frequency and intensity). However, there is not evidence in the literature about the influence of the type of chronic stress in cardiovascular changes evoked by repeated exposure to stress events. In this way, an aim of the present study is to compare the consequences of two protocols of chronic stress (variable chronic stress and repeated restraint stress) on cardiovascular function.Other propose of the present study is to investigate the influence of the ontogeny and sexual dimorphism in consequences of chronic stress on cardiovascular function. It has demonstrated a vulnerability of adolescents and individuals and animals of female sex to behavioral and neuroendocrine consequences of stress. However, the effect of chronic stress on cardiovascular function of adolescents and females was poorly investigated. Thus, the aims in the present study are: 1) to compare, in adult male rats, the effect of the chronic variable stress and the repeated exposure to restraint stress on cardiovascular function; 2) to compare, in adult female rats ovariectomized and SHAM, the effect of the chronic variable stress and the repeated exposure to restraint stress on cardiovascular function, and to investigate whether effects in these animals are different from those observed in adult male rats; and 3) to compare, in adolescents male rats, the effect of the chronic variable stress and the repeated exposure to restraint stress on cardiovascular function, and to investigate whether effects in these animals last until adult phase and are different from those observed in adult male rats. For evaluation of cardiovascular function, we will investigate the effect of the chronic stress protocols on basal parameters of arterial pressure and heart rate, baroreflex activity, vascular reactivity to vasoactive agents and the cardiac autonomic balance. (AU)

Scientific publications

(19)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, LEANDRO A.; ALMEIDA, JEFERSON; GOMES-DE-SOUZA, LUCAS; BENINI, RICARDO; CRESTANI, CARLOS C.. CRF1 and CRF2 receptors in the bed nucleus of stria terminalis differently modulate the baroreflex function in unanesthetized rats. European Journal of Neuroscience, v. 46, n. 2, p. 1805-1812, JUL 2017. (12/14376-0, 15/05922-9)

DUARTE, JOSIANE O.; GOMES, KARINA S.; NUNES-DE-SOUZA, RICARDO L.; CRESTANI, CARLOS C.. Role of the lateral preoptic area in cardiovascular and neuroendocrine responses to acute restraint stress in rats. Physiology & Behavior, v. 175, p. 16-21, JUN 1 2017. (12/14376-0, 15/05922-9, 12/01888-2)

ENGI, SHEILA A.; CRUZ, FABIO C.; LEAO, RODRIGO M.; SPOLIDORIO, LUIS C.; PLANETA, CLEOPATRA S.; CRESTANI, CARLOS C.. Cardiovascular Complications following Chronic Treatment with Cocaine and Testosterone in Adolescent Rats. PLoS One, v. 9, n. 8, AUG 14 2014. (12/14376-0, 10/16192-8)

DUARTE, JOSIANE O.; CRUZ, FABIO C.; LEAO, RODRIGO M.; PLANETA, CLEOPATRA S.; CRESTANI, CARLOS C.. Stress Vulnerability During Adolescence: Comparison of Chronic Stressors in Adolescent and Adult Rats. PSYCHOSOMATIC MEDICINE, v. 77, n. 2, p. 186-199, FEB-MAR 2015. (12/14376-0)

ALMEIDA, JEFERSON; DUARTE, JOSIANE O.; OLIVEIRA, LEANDRO A.; CRESTANI, CARLOS C.. Effects of nitric oxide synthesis inhibitor or fluoxetine treatment on depression-like state and cardiovascular changes induced by chronic variable stress in rats. STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS, v. 18, n. 4, p. 462-474, 2015. (12/14376-0)

BARRETTO-DE-SOUZA, LUCAS; ADAMI, MARIANE B.; OLIVEIRA, LEANDRO A.; GOMES-DE-SOUZA, LUCAS; DUARTE, JOSIANE O.; ALMEIDA, JEFERSON; CRESTANI, CARLOS C.. Nitric oxide-cGMP-PKG signaling in the bed nucleus of the stria terminalis modulates the cardiovascular responses to stress in male rats. European Neuropsychopharmacology, v. 28, n. 1, p. 75-84, JAN 2018. (12/14376-0, 15/05922-9)

CONSTANTINO, PAULA B.; DIONISIO, THIAGO J.; DUCHATSCH, FRANCINE; HERRERA, NAIARA A.; DUARTE, JOSIANE O.; SANTOS, CARLOS F.; CRESTANI, CARLOS C.; AMARAL, SANDRA L.. Exercise attenuates dexamethasone-induced hypertension through an improvement of baroreflex activity independently of the renin-angiotensin system. Steroids, v. 128, p. 147-154, DEC 2017. (14/23050-6, 12/19722-3, 12/14376-0, 11/21522-0, 14/18177-7)

CRESTANI, CARLOS C.. Adolescent vulnerability to cardiovascular consequences of chronic emotional stress: Review and perspectives for future research. NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS, v. 74, n. B, SI, p. 466-475, MAR 2017. (12/14376-0, 15/05922-9)

GOUVEIA, MARIANNA K.; MIGUEL, TARCISO T.; BUSNARDO, CRISTIANE; SCOPINHO, AMERICA A.; CORREA, FERNANDO M. A.; NUNES-DE-SOUZA, RICARDO L.; CRESTANI, CARLOS C.. Dissociation in control of physiological and behavioral responses to emotional stress by cholinergic neurotransmission in the bed nucleus of the stria terminalis in rats. Neuropharmacology, v. 101, p. 379-388, FEB 2016. (12/14376-0, 13/01283-6, 15/05922-9)

ENGI, SHEILA A.; PLANETA, CLEOPATRA S.; CRESTANI, CARLOS C.. Effect of Voluntary Ethanol Consumption Combined with Testosterone Treatment on Cardiovascular Function in Rats: Influence of Exercise Training. PLoS One, v. 11, n. 1, JAN 13 2016. (13/09715-2, 12/14376-0, 12/14723-1)

COSTA-FERREIRA, WILLIAN; VIEIRA, JONAS O.; ALMEIDA, JEFERSON; GOMES-DE-SOUZA, LUCAS; CRESTANI, CARLOS C.. Involvement of Type 1 Angiontensin II Receptor (AT(1)) in Cardiovascular Changes Induced by Chronic Emotional Stress: Comparison between Homotypic and Heterotypic Stressors. FRONTIERS IN PHARMACOLOGY, v. 7, AUG 18 2016. (12/14376-0, 15/05922-9)

ADAMI, MARIANE B.; BARRETTO-DE-SOUZA, LUCAS; DUARTE, JOSIANE O.; ALMEIDA, JEFERSON; CRESTANI, CARLOS C.. Both N-methyl-D-aspartate and non-N-methyl-D-aspartate glutamate receptors in the bed nucleus of the stria terminalis modulate the cardiovascular responses to acute restraint stress in rats. JOURNAL OF PSYCHOPHARMACOLOGY, v. 31, n. 6, p. 674-681, JUN 2017. (12/14376-0, 15/05922-9)

CRUZ, FABIO C.; DUARTE, JOSIANE O.; LEAO, RODRIGO M.; HUMMEL, LUIZ F. V.; PLANETA, CLEOPATRA S.; CRESTANI, CARLOS C.. Adolescent vulnerability to cardiovascular consequences of chronic social stress: Immediate and long-term effects of social isolation during adolescence. DEVELOPMENTAL NEUROBIOLOGY, v. 76, n. 1, p. 34-46, JAN 2016. (12/14376-0)

VIEIRA, JONAS O.; DUARTE, JOSIANE O.; COSTA-FERREIRA, WILLIAN; MORAIS-SILVA, GESSYNGER; MARIN, MARCELO T.; CRESTANI, CARLOS C.. Sex differences in cardiovascular, neuroendocrine and behavioral changes evoked by chronic stressors in rats. PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY, v. 81, p. 426-437, FEB 2 2018. (12/14376-0, 15/05922-9)

DUARTE, JOSIANE O.; PLANETA, CLEOPATRA S.; CRESTANI, CARLOS C.. Immediate and long-term effects of psychological stress during adolescence in cardiovascular function: Comparison of homotypic vs heterotypic stress regimens. INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, v. 40, p. 52-59, FEB 2015. (12/14376-0)

OLIVEIRA, LEANDRO A.; POLLO, TACIANA R. S.; ROSA, ELINEIA A.; DUARTE, JOSIANE O.; XAVIER, CARLOS H.; CRESTANI, CARLOS C.. Both Prelimbic and Infralimbic Noradrenergic Neurotransmissions Modulate Cardiovascular Responses to Restraint Stress in Rats. FRONTIERS IN PHYSIOLOGY, v. 12, AUG 12 2021. (19/24478-3, 12/14376-0)

OLIVEIRA, LEANDRO A.; CARVALHO, IVY I.; KUROKAWA, RENATA Y.; DUARTE, JOSIANE DE O.; BUSNARDO, CRISTIANE; CRESTANI, CARLOS C.. Differential roles of prelimbic and infralimbic cholinergic neurotransmissions in control of cardiovascular responses to restraint stress in rats. Brain Research Bulletin, v. 181, p. 8-pg., 2022-02-07. (19/24478-3, 12/14376-0)

DUARTE, JOSIANE O.; PLANETA, CLEOPATRA S.; CRESTANI, CARLOS C.. Vulnerability and resilience to cardiovascular and neuroendocrine effects of stress in adult rats with historical of chronic stress during adolescence. Life Sciences, v. 318, p. 11-pg., 2023-02-15. (12/14376-0, 15/05922-9, 19/24478-3)

CRESTANI, CARLOS C.. Adolescent vulnerability to cardiovascular consequences of chronic emotional stress: Review and perspectives for future research. NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS, v. 74, p. 10-pg., 2017-03-01. (12/14376-0, 15/05922-9)