Sugarcane energetic balance: a systems approach towards understanding regulation of sucrose metabolism and sugar signaling

• Grant number: 08/58031-0
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
• Start date: August 01, 2009
• End date: October 31, 2013
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Principal Investigator: Renato Vicentini dos Santos
• Grantee: Renato Vicentini dos Santos
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 13/05171-8 - The development of a sugar cane reference transcriptome: evolutionary aspects and its application to the sucrose metabolism knowledge, BP.PD; 12/06539-6 - EVOLUTIONARY RELATIONSHIPS BETWEEN ncRNAs AND ORPHANS OF GENES OF Arabidopsis thaliana AND Saccharum officinarum, BP.MS; 11/16450-0 - Allosteric regulation of sucrose metabolism in sugarcane, BP.MS; 09/09694-0 - Sugarcane energetic balance: a systems approach towards understanding regulation of sucrose metabolism and sugar signaling, BP.JP.BIOEN

Abstract

Sugarcane research has identified and characterized a suite of proteins involved in carbon biosynthesis and sugar sensing. However, current results towards understanding sucrose biosynthesis and accumulation have fallen short of expectations. The molecular mechanisms responsible for the cross talk between these different regulatory and signaling pathways and their diversification in plants still need to be further elucidated to better understand plant growth patterns and biomass production. We are only beginning to produce the detailed gene expression data needed for understanding the network of interactions at a molecular level. To address the rate of gene discovery, high-throughput approaches have being developed for biological experimentation and relevant biological questions regarding gene, protein interactions or networks of biological process can now be addressed. Here, we propose to develop a research approach which integrates molecular and systems biology to improve the knowledge about carbohydrates biosynthesis and sugar regulatory signaling in sugarcane. In this research project we will elaborate ways to apply analyses of regulatory network and dynamic metabolic models in molecular and genetic data of sugarcane related with sucrose biosynthesis, and define the diversification of glucose and sucrose-induced gene expression programs among angiosperms. We expected that the models captures the regulation of many sugarcane genetic components and anticipate that the data will improve our view of sugar signaling in plants. Simulations of our models will provide an efficient tool for the identification of candidate to genetic manipulations that have the best chance to promote increase in sucrose content and for the prioritization of future analyzes. The results will be integrated into databases that could feed projects related to biomass and bioenergy research. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VICENTINI, R.; DEL BEM, L. E. V.; VAN SLUYS, M. A.; NOGUEIRA, F. T. S.; VINCENTZ, M.. Gene Content Analysis of Sugarcane Public ESTs Reveals Thousands of Missing Coding-Genes and an Unexpected Pool of Grasses Conserved ncRNAs. TROPICAL PLANT BIOLOGY, v. 5, n. 2, p. 199-205, JUN 2012. (08/52071-0, 08/09105-1, 08/58031-0)

ALVES, LEONARDO CARDOSO; PORTILLA LLERENA, JUAN PABLO; MAZZAFERA, PAULO; VICENTINI, RENATO. Diel oscillations in cell wall components and soluble sugars as a response to short-day in sugarcane (Saccharum sp.). BMC PLANT BIOLOGY, v. 19, MAY 23 2019. (08/58031-0)

VICENTINI, RENATO; BOTTCHER, ALEXANDRA; BRITO, MICHAEL DOS SANTOS; DOS SANTOS, ADRIANA BROMBINI; CRESTE, SILVANA; DE ANDRADE LANDELL, MARCOS GUIMARAES; CESARINO, IGOR; MAZZAFERA, PAULO. Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content. PLoS One, v. 10, n. 8, AUG 4 2015. (08/58035-6, 08/58031-0)

DE SETTA, NATHALIA; MONTEIRO-VITORELLO, CLAUDIA BARROS; METCALFE, CUSHLA JANE; QUEIROGA CRUZ, GUILHERME MARCELO; DEL BEM, LUIZ EDUARDO; VICENTINI, RENATO; SILVEIRA NOGUEIRA, FABIO TEBALDI; CAMPOS, ROBERTA ALVARES; NUNES, SIDENY LIMA; GASPERAZZO TURRINI, PAULA CRISTINA; et al. Building the sugarcane genome for biotechnology and identifying evolutionary trends. BMC Genomics, v. 15, JUN 30 2014. (10/05591-9, 08/54201-9, 08/52074-0, 08/58031-0, 08/52197-4, 08/52146-0)

BRITO, MICHAEL SANTOS; NOBILE, PAULA MACEDO; BOTTCHER, ALEXANDRA; DOS SANTOS, ADRIANA BROMBINI; CRESTE, SILVANA; ANDRADE DE LANDELL, MARCOS GUIMARES; VINCENTZ, MICHEL; VICENTINI, RENATO; MAZZAFERA, PAULO. Expression Profile of Sugarcane Transcription Factor Genes Involved in Lignin Biosynthesis. TROPICAL PLANT BIOLOGY, v. 8, n. 1-2, p. 19-30, JUN 2015. (08/58035-6, 08/58031-0)

CARDOSO-SILVA, CLAUDIO BENICIO; COSTA, ESTELA ARAUJO; MANCINI, MELINA CRISTINA; ALMEIDA BALSALOBRE, THIAGO WILLIAN; COSTA CANESIN, LUCAS EDUARDO; PINTO, LUCIANA ROSSINI; CARNEIRO, MONALISA SAMPAIO; FRANCO GARCIA, ANTONIO AUGUSTO; DE SOUZA, ANETE PEREIRA; VICENTINI, RENATO. De Novo Assembly and Transcriptome Analysis of Contrasting Sugarcane Varieties. PLoS One, v. 9, n. 2, FEB 11 2014. (08/52197-4, 08/58031-0)