Immunological response evaluation in patients with Endometriosis

• Grant number: 05/01218-3
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2005
• End date: October 31, 2006
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Maurício Simões Abrão
• Grantee: Maurício Simões Abrão
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Endometriosis is characterized by the presence of endometriotic tissue in ectopic sites and the participation of the immune system has been studied in different aspects of this disease. The Th1/Th2 response to the antigenic stilmulus involves distinct cytokynes, and it unleashes celular (Th1) or humoral (Th2) response. Amongst the cytokynes involved in this process, it is possible to exceed tumor necrosis factor-alpha, interferon-gamma, interleukins 1,4,6,10,12 and 18, besides natural killer cells. Concerning humoral response, one may also consider the presence of autoantibodies like antinuclear factor, anticardiolipin antibodies, antiphosphatidylserin antibodies, antithyreoglobulin antibodies and antiperoxidase antibodies. Patients and methods: The research, a case-control study, has been approved by the Ethic and Research Committee from the Hospital das Clinicas, School of Medicine, University of São Paulo (Protocol n 601/03). It will take place at Endometriosis Division, Gynecologic Clinic, Hospital das Clinicas, School of Medicine, University of São Paulo. During one year, all patients submitted to a surgical evaluation to verify the endometriosis occurrence will be selected. The selected cases will be separated in two groups, with (A) or without (B) endometriosis, based on histological exam. All cases will have surgical procedures through laparoscopy. Previously to the onset of the laparoscopic surgery, 30ml of peripheral venous blood will be collected. Sequentially, all the peritoneal fluid will be collected from the abdominal cavity. The Natural Killer cells identification and counting will be performed immediately, when the sample arrive at the laboratory. The remaining blood and peritoneal fluid will be stored at -80o C. We will obtain clinical data from all patients, regarding endometriosis symptoms [...]. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

EJZENBERG, DANI; PODGAEC, SERGIO; DIAS, JR., JOAO ANTONIO; DE OLIVEIRA, RICARDO MANOEL; BARACAT, EDMUND CHADA; ABRAO, MAURICIO SIMOES. Measurement of Serum and Peritoneal Levels of Amyloid Protein A and Their Importance in the Diagnosis of Pelvic Endometriosis. JOURNAL OF REPRODUCTIVE MEDICINE, v. 58, n. 9-10, p. 411-416, SEP-OCT 2013. (05/01218-3)

SERGIO PODGAEC; JOÃO ANTONIO DIAS JUNIOR; CHARLES CHAPRON; RICARDO MANOEL DE OLIVEIRA; EDMUND CHADA BARACAT; MAURÍCIO SIMÕES ABRÃO. Resposta imunológica Th1 e Th2 relacionada à endometriose pélvica. Revista da Associação Médica Brasileira, v. 56, n. 1, p. 92-98, 2010-00-00. (05/01218-3)

DIAS, JOAO ANTONIO, JR.; PODGAEC, SERGIO; DE OLIVEIRA, RICARDO MANOEL; CARNEVALE MARIN, MARIA LUCIA; BARACAT, EDMUND CHADAD; ABRAO, MAURICIO SIMOES. Patients With Endometriosis of the Rectosigmoid Have a Higher Percentage of Natural Killer Cells in Peripheral Blood. JOURNAL OF MINIMALLY INVASIVE GYNECOLOGY, v. 19, n. 3, p. 8-pg., 2012-05-01. (05/01218-3)