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Standardization and implantation of the real time PCR for detection and quantification of hepatitis B virus


Hepatitis B virus (HBV) is the major cause of chronic hepatitis, cirrhosis and hepatocellular carcinoma and, monitoring HBV DNA levels in serum, plays an important role in the management of the infection. The aim of this study is to standard a sensitive and reproducible real-time PCR, based on TaqMan technology, for the detection and quantification of hepatitis B virus in serum of infected patients. Two hundred sera from chronic hepatitis B patients will be analyzed by real time PCR and comparing with Amplicor Monitor™ Test (Roche Laboratories). We also will employ different HBV genotypes to compare the performance of the real time PCR. Real time PCR will be useful for evaluating response to lamivudine, to detect pre-core mutants and to monitoring occult hepatitis B infection. Institute Adolfo Lutz is an important laboratory of public health and technological improvement is always needed. (AU)

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(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE TORRES SANTOS, ANA PAULA; LEVI, JOSE EDUARDO; LEMOS, MARCILIO FIGUEIREDO; CALUX, SAMIRA JULIEN; OBA, ISABEL TAKANO; MOREIRA, REGINA CELIA. An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA. Memórias do Instituto Oswaldo Cruz, v. 111, n. 2, p. 134-140, . (09/53086-4)

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