Evaluation of DNA damage in Anesthesiologists

• Grant number: 13/05084-8
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2013
• End date: December 31, 2015
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Leandro Gobbo Braz
• Grantee: Leandro Gobbo Braz
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• City of the host institution: Botucatu
• Associated researchers: Mariana Gobbo Braz

Abstract

The human monitoring has been used as a useful tool to identify and quantify the risk related to harmful effects in health. There is a few data in Brazil regarding the monitoring of occupational exposure to waste anesthetic gases, without national data of waste anesthetic gases determined in operating room (OR). This proposal aims to determine the concentrations of waste anesthetic gases in the OR and evaluate DNA damage in exposed medical personnel. The study will be performed in the OR of the Hospital of Botucatu Medical School - UNESP and in 60 subjects: 30 anesthesiologists exposure to waste anesthetic gases and 30 physicians without occupational exposure, matched by age, gender and lifestyle. The concentrations of isoflurane, sevoflurane, desflurane and nitrous oxide (waste anesthetic gases) will be determined in the OR using a portable infrared spectrophotometer. Venous blood samples and buccal cells will be collected from all professionals. DNA damage evaluated in lymphocytes will be detected by comet assay and chromosomal damage by buccal micronucleus cytome assay. Taken together, data of the values of waste anesthetic gases in OR and their possible effects on genetic material will be assessed in anesthesiologists. This knowledge will certainly contribute to future preventive actions, adequating the concentrations of waste anesthetic gases to international standards and reducing the possible damage into the exposed professionals, with direct impact on public health. (AU)

Scientific publications

(7)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

BRAZ, MARIANA G.; SILVA, MARIANE A. P.; FIGUEIREDO, DRIELLE B. S.; AUN, ALINE G.; MARQUES, LAIS S. K.; LARA, JULIANA R.; BRAZ, LEANDRO G.. Genetic instability assessed by telomere length and micronucleus in physicians exposed to anesthetics. Environmental and Molecular Mutagenesis, JUN 2020. (13/21130-0, 13/05084-8, 16/15559-1)

LEANDRO GOBBO BRAZ; JOSÉ REINALDO CERQUEIRA BRAZ; GUILHERME APARECIDO SILVA CAVALCANTE; KÁTINA MENEGHETTI SOUZA; LORENA MENDES DE CARVALHO LUCIO; MARIANA GOBBO BRAZ. Comparação de resíduos de gases anestésicos em salas de operação com ou sem sistema de exaustão em hospital universitário brasileiro. Revista Brasileira de Anestesiologia, v. 67, n. 5, p. 516-520, 2017-10-00. (13/05084-8, 13/21130-0)

SILVA, MARIANE A. P.; FIGUEIREDO, DRIELLE B. S.; LARA, JULIANA R.; PASCHOALINOTTE, ELOISA E.; BRAZ, LEANDRO G.; BRAZ, MARIANA G.. Evaluation of genetic instability, oxidative stress, and metabolism-related gene polymorphisms in workers exposed to waste anesthetic gases. Environmental Science and Pollution Research, v. N/A, p. 15-pg., 2022-09-03. (13/21130-0, 18/20900-0, 13/05084-8)

BRAZ, MARIANA G.; SILVA, MARIANE A. P.; FIGUEIREDO, DRIELLE B. S.; AUN, ALINE G.; MARQUES, LAIS S. K.; LARA, JULIANA R.; BRAZ, LEANDRO G.. Genetic instability assessed by telomere length and micronucleus in physicians exposed to anesthetics. Environmental and Molecular Mutagenesis, v. 61, n. 8, p. 5-pg., 2020-06-24. (16/15559-1, 13/21130-0, 13/05084-8)

SOUZA, KATINA M.; BRAZ, LEANDRO G.; NOGUEIRA, FLAVIA R.; SOUZA, MARAJANE B.; BINCOLETO, LAHIS F.; AUN, ALINE G.; CORRENTE, JOSE E.; CARVALHO, LIDIA R.; BRAZ, JOSE REINALDO C.; BRAZ, MARIANA G.. Occupational exposure to anesthetics leads to genomic instability, cytotoxicity and proliferative changes. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, v. 791, p. 42-48, SEP-OCT 2016. (13/05084-8)

SILVA, MARIANE A. P.; FIGUEIREDO, DRIELLE B. S.; DE CARVALHO, LIDIA R.; BRAZ, LEANDRO G.; BRAZ, MARIANA G.. Modulation of gene expression and influence of gene polymorphisms related to genotoxicity and redox status on occupational exposure to inhaled anesthetics. INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH, v. 256, p. 10-pg., 2023-12-08. (13/21130-0, 13/05084-8, 18/20900-0)

BRAZ, MARIANA G.; SOUZA, KATINA M.; LUCIO, LORENA M. C.; DI RENZO, GIULIA C. C.; FELICIANO, LUCIANA M.; MARCONDES, JOAO PAULO C.; OLIVER CHEN, C. -Y.; BRAZ, JOSE REINALDO C.; BRAZ, LEANDRO G.. Detrimental effects detected in exfoliated buccal cells from anesthesiology medical residents occupationally exposed to inhalation anesthetics: An observational study. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 832, p. 61-64, AUG 2018. (13/21130-0, 13/05084-8)