Molecular characterization of plasmids in Brazilian clinical isolates carrying qnr genes

• Grant number: 12/18741-4
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2013
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Luciene Andrade da Rocha Minarini
• Grantee: Luciene Andrade da Rocha Minarini
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated researchers: Ana Cristina Gales

Abstract

It is very important to know how microorganisms acquire and express resistance phenotype to promote the development of effective strategy for regulating the use of antimicrobial agents commonly used in Brazil. Therefore, the research project was designed to investigate plasmideal mechanisms involved in the maintenance of resistant bacterial strains at the environment. Plasmids carrying qnr determinants previously reported in bacterial strains isolated in Brazil (n = 19) will be typed and sub-typed by Multiplex PCR and Sequencing by multilocus (pMLST), molecular methods that have not been explored in Brazil, which allow to compare data obtained from strains isolated in various parts of the world. The results expand knowledge about types of plasmids involved in these resistance phenotypes, allowing the determination of the epidemiological profile of occurrence of plamideal groups higher frequency. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA RIBEIRO, AGHATA CARDOSO; BRASILEIRO DA SILVA MARTINS, WILLAMES MARCOS; DA SILVA, ADILSON ADERITO; GALES, ANA CRISTINA; GALASSE RANDO, DANIELA GONCALES; DA ROCHA MINARINI, LUCIENE ANDRADE. Exposure to sub-inhibitory ciprofloxacin and nitrofurantoin concentrations increases recA gene expression in uropathogenic Escherichia coli: The role of RecA protein as a drug target. European Journal of Pharmaceutical Sciences, v. 146, APR 15 2020. (12/18741-4)

SEGURA, WILSON DIAS; RAMOS, HAISSA PEREIRA; DE FARIA BLANC AMORIM, RENATA ESTER; DA SILVA RIBEIRO, AGHATA CARDOSO; PEREIRA, EDIMAR CRISTIANO; CAYO, RODRIGO; GALES, ANA CRISTINA; PIANTINO FERREIRA, ANTONIO J.; DA ROCHA MINARINI, LUCIENE ANDRADE. In vitro and in vivo persistence of IncN plasmids carrying qnr genes in uropathogenic Escherichia coli isolates. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 22, p. 5-pg., 2020-09-01. (12/18741-4)