Evaluation of the effects of the cytokine SOFAT on osteoblast cells and its potential on bone inflammatory diseases

• Grant number: 13/09524-2
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2013
• End date: September 30, 2015
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Marcelo Henrique Napimoga
• Grantee: Marcelo Henrique Napimoga
• Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil
• Associated researchers: Elizabeth Ferreira Martinez

Abstract

A novel activated human T cell-secreted cytokine, referred as secreted osteoclastogenic factor of activated T cells (SOFAT), that induce osteoclastogenesis in a RANKL-independent manner was recently described. We previously demonstrated that the mRNA and protein levels of SOFAT were significantly higher in the gingival tissue of the periodontitis group when compared to non-periodontitis one. In addition, SOFAT potently induced TRAP-positive multinucleated cell formation by RAW 264.7 cells as well as induced the formation of osteoclast-like cells in the periodontal ligament in mice. The present study aims to evaluate the role of this new cytokine on bone inflammatory diseases. Thus, it will be exploited the activity of this cytokine on osteoblast cells in order to evaluate if this cytokine may induce higher levels of pro-inflammatory cytokines as well as to evaluate the RANKL expression in the presence of SOFAT. Besides, it will be evaluated which sub-type of T lymphocyte is able to produce SOFAT. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NAPIMOGA, MARCELO HENRIQUE; DIAS DEMASI, ANA PAULA; JARRY, CHRISTIAN RADO; ORTEGA, MAURICIO CARDOSO; DE ARAUJO, VERA CAVALCANTI; MARTINEZ, ELIZABETH FERREIRA. In vitro evaluation of the biological effect of SOFAT on osteoblasts. International Immunopharmacology, v. 26, n. 2, p. 378-383, JUN 2015. (13/09524-2)

NAPIMOGA, MARCELO HENRIQUE; DANTAS FORMIGA, WESLLEY DANNY; ABDALLA, HENRIQUE BALLASSINI; TRINDADE-DA-SILVA, CARLOS ANTONIO; VENTURIN, CAMILA MOTTA; MARTINEZ, ELIZABETH FERREIRA; ROSSANEIS, ANA CAROLINA; VERRI JR, WALDICEU A.; CLEMENTE-NAPIMOGA, JULIANA TRINDADE. Secreted Osteoclastogenic Factor of Activated T Cells (SOFAT) Is Associated With Rheumatoid Arthritis and Joint Pain: Initial Evidences of a New Pathway. FRONTIERS IN IMMUNOLOGY, v. 11, JUL 28 2020. (13/09524-2, 17/22334-9)

JARRY, CHRISTIAN R.; MARTINEZ, ELIZABETH F.; PERUZZO, DAIANE C.; CARREGARO, VANESSA; SACRAMENTO, LAIS A.; ARAUJO, VERA C.; WEITZMANN, M. NEALE; NAPIMOGA, MARCELO H.. Expression of SOFAT by T- and B-lineage cells may contribute to bone loss. MOLECULAR MEDICINE REPORTS, v. 13, n. 5, p. 4252-4258, MAY 2016. (13/09524-2)