Evaluation of the capacity of bacteriophage 933W to transduce the Stx gene to host cells in vivo: impacts on the development of vaccines against hemolytic uremic syndrome

• Grant number: 13/20635-0
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• Start date: February 02, 2014
• End date: April 01, 2014
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Luis Carlos de Souza Ferreira
• Grantee: Luis Carlos de Souza Ferreira
• Visiting researcher: Leticia Veronica Bentancor
• Visiting researcher institution: Universidad Nacional de Quilmes (UNQ), Argentina
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

We want to evaluate if the bacteriophage 933W is able to transduce host cells in vivo. These results could impact on the STEC-HUS comprehension, since local eukaryotic cells transduced with and/or infected by bacteriophage encoding Stx2, could be an alternative source to produce Stx2 and should be helpful to design a new vaccine approach against the bacteriophage implicated in Stx production. (AU)