Clonal evaluation of Clostridium perfringens strains isolated from humans and animals by Multilocus sequence typing (MLST)

• Grant number: 13/17739-9
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2014
• End date: January 31, 2016
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Mario Júlio Ávila-Campos
• Grantee: Mario Júlio Ávila-Campos
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated researchers: Viviane Nakano

Abstract

Clostridium perfringens is considered a ubiquitous microorganism in the environment and in the vertebrate's intestinal tract, but this bacterium can cause severe diseases in human or animal. The goal of this study is to determine the toxinotyping and clonal similarity analysis of 40 strains of C. perfringens isolated from fecal samples of children and chickens with necrotizing enterocolitis by using the Multilocus Sequence Typing method (MLST). In this study we will use seven housekeeping genes: D-alanine D-alanine ligase (ddlA), superoxide dismutase (sod), deoxyuridine triphosphate (dut), glycerol kinase (glpK), deoxiguanilato kinase (gmK), and recombinase (recA), as well as the virulence gene plc for phospholipase C - alpha toxin. The results may provide microbiological, genetic and molecular data to better understand the epidemiology, virulence, and the clone distribution of this important anaerobic microorganism. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES, MIRIAM R.; IGNACIO, ALINE; RODRIGUES, VIVIANE A. A.; GROPPO, FRANCISO C.; CARDOSO, ARY L.; AVILA-CAMPOS, MARIO J.; NAKANO, VIVIANE. Alterations of Intestinal Microbiome by Antibiotic Therapy in Hospitalized Children. MICROBIAL DRUG RESISTANCE, v. 23, n. 1, p. 56+, JAN 2017. (13/17739-9)

LLANCO, LUIS A.; NAKANO, VIVIANE; AVILA-CAMPOS, MARIO J.. Sialidase Production and Genetic Diversity in Clostridium perfringens Type A Isolated from Chicken with Necrotic Enteritis in Brazil. Current Microbiology, v. 70, n. 3, p. 330-337, MAR 2015. (09/03792-0, 13/17739-9)

LLANCO, LUIS A.; NAKANO, VIVIANE; DE MORAES, CLAUDIA T. P.; PIAZZA, ROXANE M. F.; AVILA-CAMPOS, MARIO J.. Adhesion and invasion of Clostridium perfringens type A into epithelial cells. Brazilian Journal of Microbiology, v. 48, n. 4, p. 5-pg., 2017-10-01. (13/17739-9)

LLANCO, LUIS A.; NAKANO, VIVIANE; DE MORAES, CLAUDIA T. P.; PIAZZA, ROXANE M. F.; AVILA-CAMPOS, MARIO J.. Adhesion and invasion of Clostridium perfringens type A into epithelial cells. Brazilian Journal of Microbiology, v. 48, n. 4, p. 764-768, OCT-DEC 2017. (13/17739-9)

MERINO, VICTOR R.; NAKANO, VIVIANE; DELANNOY, SABINE; FACH, PATRICK; ALBERCA, GABRIELA G. F.; FARFAN, MAURICIO J.; PIAZZA, ROXANE M. F.; AVILA-CAMPOS, MARIO J.. Prevalence of Enteropathogens and Virulence Traits in Brazilian Children With and Without Diarrhea. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 10, SEP 25 2020. (17/14821-7, 13/17739-9)

IGNACIO, A.; FERNANDES, M. R.; RODRIGUES, V. A. A.; GROPPO, F. C.; CARDOSO, A. L.; AVILA-CAMPOS, M. J.; NAKANO, V.. Correlation between body mass index and faecal microbiota from children. Clinical Microbiology and Infection, v. 22, n. 3, MAR 2016. (12/10659-7, 13/17739-9)