| Full text | |
| Author(s): |
Ignacio, A.
[1]
;
Fernandes, M. R.
[1]
;
Rodrigues, V. A. A.
[1]
;
Groppo, F. C.
[2]
;
Cardoso, A. L.
[3]
;
Avila-Campos, M. J.
[1]
;
Nakano, V.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Anaerobe Lab, Av Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, SP - Brazil
[2] Univ Estadual Campinas, Campinas, SP - Brazil
[3] Univ Sao Paulo, Fac Med, Inst Children, Sao Paulo, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Clinical Microbiology and Infection; v. 22, n. 3 MAR 2016. |
| Web of Science Citations: | 34 |
| Abstract | |
Childhood obesity is an increasing problem at the global level and considered as a risk factor for obesity development and the associated comorbidities in adult life. In this study, the occurrence of Bacteroides fragilis group, Clostridium spp., Bifidobacterium spp. and Escherichia coli in 84 faecal samples from 30 obese, 24 overweight and 30 lean children was verified by culture technique and quantitative determination by quantitative PCR. In addition, Lactobacillus spp. and Methanobrevibacter smithii were also analysed. A correlation between the body mass index (BMI) and these bacteria was sought. Bacteroides vulgatus, Clostridium perfringens and Bifidobacterium adolescentis were most prevalent in all samples evaluated by culture-method. The B. fragilis group were found at high concentrations in obese and overweight children when compared with the lean ones (p0.015). The obese and overweight children harboured higher numbers of Lactobacillus spp. than lean children (p0.022). The faecal concentrations of the B. fragilis group (r=0.24; p0.026) and Lactobacillus spp. (r=0.44; p0.002) were positively correlated with BMI. Bifidobacterium spp. were found in higher numbers in the lean group than the overweight and obese ones (p0.042). Furthermore, a negative correlation between BMI and Bifidobacterium spp. copy number (r= -0.22;p0.039) was observed. Our findings show some difference in the intestinal microbial ecosystem of obese children compared with the lean ones and a significant association between number of Lactobacillus spp. and B. fragilis group and BMI. Clinical Microbiology and Infection (C) 2015 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 12/10659-7 - Evaluation of some microorganisms of the endogenous intestinal microbiota of children with normal weight, obese and overweight in school-age |
| Grantee: | Aline Ignacio Silvestre da Silva |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 13/17739-9 - Clonal evaluation of Clostridium perfringens strains isolated from humans and animals by Multilocus sequence typing (MLST) |
| Grantee: | Mario Júlio Ávila-Campos |
| Support Opportunities: | Regular Research Grants |