Impact of gastroplasty on salivary composition and cytomorphology of oral mucosa: follow-up three and six months

• Grant number: 16/10940-9
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): September 01, 2017
• Effective date (End): October 31, 2020
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Paula Midori Castelo Ferrua
• Grantee: Maria Carolina Salomé Marquezin
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

The aim of this study is to evaluate the impact of gastroplasty in salivary physicochemical and microbiological composition, concentration of sulfurated compounds volatile related to halitosis and cytomorphology of the oral mucosa of 60 obese adults (18-40 years of age) undergoing gastroplasty (Capella technique) evaluated before and 3 and 6 months after surgery. Thirty normal weight (body mass index between 18.5 and 24.9 kg/m2), matched for gender and age, will be part of the control group. The physical examination includes measurements of height, weight, fat mass and skeletal muscle mass through a digital stadiometer and bioelectrical impedance analysis. DMFT and Community Periodontal Index will verify the oral health status. The self- perception of oral health- related quality of life will be verify using OHIP-14 questionnaire. Salivary amylase, total protein, calcium, uric acid and phosphorus concentrations will be determined in stimulated and unstimulated saliva by automated technique. Detection panel via Luminex will analyze salivary cytokines. Salivary DNA samples will be purified and subjected to quantitative PCR reactions (qPCR) in order to determine the proportion of Selenomonas noxia, Bifidobacteria, Streptococcus mutans and Prevotella intermedia in relation to the total bacterial load. The concentration of sulfurated compounds volatile [sulfhydrate (SH2), methyl mercaptan (CH3SH) and dimethyl sulfide (CH3)2S] related to halitosis will be verified using a gas chromatograph, Oral Chroma®. Micronucleus test will analyze oral mucosa cells. The description of the study variables and their correlation will be analyzed using descriptive statistics, normality test and correlation tests (Pearson or Spearman). Paired t-test/Wilcoxon will verify the changes resulting from bariatric surgery; unpaired t-test/Mann Whitney will be used for comparisons between groups. A multiple logistic regression model will verify the association of the variables under study with surgery (gastroplasty), controlling for confounders (± = 0.05).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROSAR, JOAO VICENTE; SALOME MARQUEZIN, MARIA CAROLINA; PIZZOLATO, AIANNE SOUTO; KOBAYASHI, FERNANDA YUKIE; BUSSADORI, SANDRA KALIL; PEREIRA, LUCIANO JOSE; CASTELO, PAULA MIDORI. Identifying predictive factors for sleep bruxism severity using clinical and polysomnographic parameters: a principal component analysis. JOURNAL OF CLINICAL SLEEP MEDICINE, v. 17, n. 5, p. 949-956, MAY 1 2021. (17/26400-6, 16/10940-9)

MARQUEZIN, MARIA CAROLINA SALOME; CHAVES-JUNIOR, SAMUEL DE CARVALHO; RASERA, JR., IRINEU; PACHECO, ELISANE ROSSIN PESSOTTI; GAVIAO, MARIA BEATRIZ DUARTE; LAMY, ELSA; CASTELO, PAULA MIDORI. Oral Health and Nutritional Characteristics of Adults With Morbid Obesity: A Multivariate Analysis. FRONTIERS IN NUTRITION, v. 7, NOV 20 2020. (17/26400-6, 16/10940-9)