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Clonal evaluation of Clostridium perfringens strains isolated from humans and animals by Multilocus sequence typing (MLST)

Grant number: 13/17739-9
Support type:Regular Research Grants
Duration: February 01, 2014 - January 31, 2016
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Mario Júlio Ávila-Campos
Grantee:Mario Júlio Ávila-Campos
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers:Viviane Nakano


Clostridium perfringens is considered a ubiquitous microorganism in the environment and in the vertebrate's intestinal tract, but this bacterium can cause severe diseases in human or animal. The goal of this study is to determine the toxinotyping and clonal similarity analysis of 40 strains of C. perfringens isolated from fecal samples of children and chickens with necrotizing enterocolitis by using the Multilocus Sequence Typing method (MLST). In this study we will use seven housekeeping genes: D-alanine D-alanine ligase (ddlA), superoxide dismutase (sod), deoxyuridine triphosphate (dut), glycerol kinase (glpK), deoxiguanilato kinase (gmK), and recombinase (recA), as well as the virulence gene plc for phospholipase C - alpha toxin. The results may provide microbiological, genetic and molecular data to better understand the epidemiology, virulence, and the clone distribution of this important anaerobic microorganism. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LLANCO, LUIS A.; NAKANO, VIVIANE; DE MORAES, CLAUDIA T. P.; PIAZZA, ROXANE M. F.; AVILA-CAMPOS, MARIO J. Adhesion and invasion of Clostridium perfringens type A into epithelial cells. Brazilian Journal of Microbiology, v. 48, n. 4, p. 764-768, OCT-DEC 2017. Web of Science Citations: 1.
FERNANDES, MIRIAM R.; IGNACIO, ALINE; RODRIGUES, VIVIANE A. A.; GROPPO, FRANCISO C.; CARDOSO, ARY L.; AVILA-CAMPOS, MARIO J.; NAKANO, VIVIANE. Alterations of Intestinal Microbiome by Antibiotic Therapy in Hospitalized Children. MICROBIAL DRUG RESISTANCE, v. 23, n. 1, p. 56+, JAN 2017. Web of Science Citations: 2.
Adhesion and invasion of Clostridium perfringens type A into epithelial cells. Brazilian Journal of Microbiology, v. 48, n. 4, p. -, Dez. 2017.
IGNACIO, A.; FERNANDES, M. R.; RODRIGUES, V. A. A.; GROPPO, F. C.; CARDOSO, A. L.; AVILA-CAMPOS, M. J.; NAKANO, V. Correlation between body mass index and faecal microbiota from children. Clinical Microbiology and Infection, v. 22, n. 3 MAR 2016. Web of Science Citations: 32.
LLANCO, LUIS A.; NAKANO, VIVIANE; AVILA-CAMPOS, MARIO J. Sialidase Production and Genetic Diversity in Clostridium perfringens Type A Isolated from Chicken with Necrotic Enteritis in Brazil. Current Microbiology, v. 70, n. 3, p. 330-337, MAR 2015. Web of Science Citations: 5.

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