Virulence factors of Gram negative bacilli and its role on peritoneal dialysis-related peritonitis outcome

• Grant number: 13/21379-8
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2014
• End date: March 31, 2016
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Pasqual Barretti
• Grantee: Pasqual Barretti
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Maria de Lourdes Ribeiro de Souza da Cunha ; Rodrigo Tavanelli Hernandes

Abstract

Gram-negative bacilli (GNB) peritonitis is a major complication of peritoneal dialysis (PD) and significant percentage of these infections have more severe clinical course and greater technique failure rate than those caused by Gram-positive cocci, particularly coagulase-negative staphylococci. Although intrinsic virulence factors of Pseudomonas spp, bacterial resistance, biofilm formation, among other factors have been reported as responsible for the less favorable clinical course of these infections there are limited amount of available evidence on factors or conditions associated with the clinical course and prognosis of these infections. The objective of this project is to describe episodes of peritonitis BGN occurred in patients treated by PD in single university center in the last 15 years and to evaluate the influence of the microbiological characteristics of the causative agent, patient's clinical condition and treatment onm peritonitis outcome. In strains of GNB , it will be tested the presence of virulence markers by genotypic methods; in vitro susceptibility to antimicrobial agents; determined the production of biofilm and analyzed the clonal profile of most prevalent bacteria using pulsed field gel electrophoresis (PFGE). From the patients data and of causative microorganism it will be studied the associations between the microbiological characteristics and the clinical outcome and peritonitis complications, as well as the associations between demographic and clinical characteristics of the patients and antimicrobial treatment and the outcome of these infections. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORO LIMA DOS SANTOS, ANA CLAUDIA; HERNANDES, RODRIGO TAVANELLI; MONTELLI, AUGUSTO CEZAR; MARINHO MONTEIRO, AYDIR CECILIA; BARBOSA, THAIS ALVES; CAMARGO, CARLOS HENRIQUE; FERREIRA, ADRIANO MARTISON; MONDELLI, ALESSANDRO LIA; RIBEIRO DE SOUZA DA CUNH, MARIA DE LOURDES; BARRETTI, PASQUAL. Clinical and microbiological factors predicting outcomes of nonfermenting gram-negative bacilli peritonitis in peritoneal dialysis. SCIENTIFIC REPORTS, v. 11, n. 1, JUN 10 2021. (13/21379-8)

DIAS, REGIANE C. B.; VIEIRA, MELISSA A.; MORO, ANA C.; RIBOLLI, DANILO F. M.; MONTEIRO, AYDIR C. M.; CAMARGO, CARLOS H.; TIBA-CASAS, MONIQUE R.; SOARES, FLAVIA B.; DOS SANTOS, LUIS F.; MONTELLI, AUGUSTO C.; et al. Characterization of Escherichia coli obtained from patients undergoing peritoneal dialysis and diagnosed with peritonitis in a Brazilian centre. Journal of Medical Microbiology, v. 68, n. 9, p. 1330-1340, SEP 2019. (13/21379-8)

MORO LIMA DOS SANTOS, ANA CLAUDIA; MARINHO MONTEIRO, AYDIR CECILIA; BARBOSA, THAIS ALVES; MORAES RIBOLI, DANILO FLAVIO; CAMARGO, CARLOS HENRIQUE; FERREIRA, ADRIANO MARTISON; MONDELLI, ALESSANDRO LIA; MONTELLI, AUGUSTO CEZAR; HERNANDES, RODRIGO TAVANELLI; RIBEIRO DE SOUZA DA CUNHA, MARIA DE LOURDES; et al. Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients. PATHOGENS, v. 11, n. 2, p. 12-pg., 2022-02-01. (13/21379-8)