Molecular networks regulated by small non-coding RNAs in hepatic fibrosis process and their modulatory effect of the vasoactive peptide angiotensin-(1-7)

• Grant number: 13/21186-5
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2014
• End date: August 31, 2016
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Karen Cristiane Martinez de Moraes
• Grantee: Karen Cristiane Martinez de Moraes
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated researchers: Bruno Cogliati ; Carol Jane Wilusz ; Igor Jurisica

Abstract

Understand the fine-tunning of gene expression that keeps the organism integrity has been considered a focus of investigation in several research areas. In this context, to investigate small non-coding RNAs (sncRNAs), more precisely microRNAs (miRNAs), is considered a hot spot in science nowadays. Those small molecules modulate gene expression and studies have appointed its future use in drug development by pharmaceutical companies. In fact, in order to miRNA could be used in clinical, a detailed mechanistic function of those molecules and molecular networks must be elucidated. Actually, hepatic fibrosis is still considered a serious problem in public health and the development of new therapies are major goal. More recently studies demonstrated a potential antifibrotic activity of the vasoactive peptide Angiotensina-(1-7) [(Ang-(1-7)], which was also appointed as a strong candidate in modulatting the level and activity of the cyclooxygenase-2 (COX-2) protein. COX-2 is the major enzyme responsible for the controlling of the inflammatory process, wich directly or indirectly correlates with the fibrotic process. However, the network of molecular mechanisms in hepatic fibrosis and its modulation by the vasoactive peptide still been a matter of investigation. Based on the exposed, the identification of central elements in liver fibrotic pathway and their correlations with miRNAs will probably redirects clinical trials. For that, cellular assays, qRT-PCRs, next generation sequencing, molecular interactions validations, Western blots, RNA interference, and bioinformatic analyses will be performed. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, BRENDA DE OLIVEIRA; RAMOS, LETICIA FERRREIRA; MORAES, KAREN C. M.. Molecular interplays in hepatic stellate cells: apoptosis, senescence, and phenotype reversion as cellular connections that modulate liver fibrosis. Cell Biology International, v. 41, n. 9, p. 946-959, SEP 2017. (13/21186-5)

MIRANDA, JULIANA F.; SCARINCI, LETICIA D.; RAMOS, LETICIA F.; SILVA, CAIO M.; GONCALVES, LETICIA R.; DE MORAIS, PRISCILA F.; MALASPINA, OSMAR; MORAES, KAREN C. M.. The modulatory effect of triclosan on the reversion of the activated phenotype of LX-2 hepatic stellate cells. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, v. 34, n. 1, JAN 2020. (13/21186-5, 18/05286-3)

DA SILVEIRA, MARINA BONFOGO; LIMA, KELVIN FURTADO; DA SILVA, ANDREA RENATA; SOUZA DOS SANTOS, ROBSON AUGUSTO; MORAES, KAREN C. M.. Mir-513a-3p contributes to the controlling of cellular migration processes in the A549 lung tumor cells by modulating integrin beta-8 expression. Molecular and Cellular Biochemistry, v. 444, n. 1-2, p. 43-52, JUL 2018. (14/21645-2, 13/21186-5)

DA SILVEIRA, MARINA BONFOGO; PANSA, CAMILA CRISTIANE; MALASPINA, OSMAR; MORAES, KAREN C. M.. The functional activity of the miR-1914-5p in lipid metabolism of the hepatocarcinoma cell line HepG2: a potential molecular tool for controlling hepatic cellular migration. MOLECULAR BIOLOGY REPORTS, v. 48, n. 4, p. 3463-3474, APR 2021. (18/05286-3, 13/21186-5)

DA SILVA, CAIO MATEUS; CAETANO, FLAVIO HENRIQUE; CAMPOS PEREIRA, FRANCO DANI; MARIN MORALES, MARIA APARECIDA; SAKANE, KUMIKO KOIBUCHI; MORAES, KAREN C. M.. Cellular and molecular effects of Baccharis dracunculifolia DC and Plectranthus barbatus Andrews medicinal plant extracts on retinoid metabolism in the human hepatic stellate cell LX-2. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, v. 19, n. 1, AUG 22 2019. (13/21186-5)

GONCALVES, LETICIA ROCHA; DA SILVA, BRENDA DE OLIVEIRA; SCARINCI, LETICIA DESTEFANI; RAMOS, LETICIA FERREIRA; MORAES, KAREN C. M.. MicroRNA-1254 contributes to the controlling of pro-fibrogenic environment in LX-2 cells by modulating SMAD3 and wound repair: new insights in hepatic fibrosis. Cell Biology International, v. 43, n. 3, p. 333-343, MAR 2019. (13/21186-5)

DA SILVA, BRENDA DE OLIVEIRA; ALBERICI, LUCIANE CARLA; RAMOS, LETICIA FERREIRA; SILVA, CAIO MATEUS; DA SILVEIRA, MARINA BONFOGO; DECHANT, CARLOS R. P.; FRIEDMAN, SCOTT L.; SAKANE, KUMIKO KOIBUCHI; GONCALVES, LETICIA ROCHA; MORAES, KAREN C. M.. Altered global microRNA expression in hepatic stellate cells LX-2 by angiotensin-(1-7) and miRNA-1914-5p identification as regulator of pro-fibrogenic elements and lipid metabolism. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, v. 98, p. 137-155, MAY 2018. (09/07671-2, 13/21186-5, 10/17259-9)

TAFURT-CARDONA, Y.; SUARES-ROCHA, P.; SILVA, B. O.; MORAES, K. C. M.; MARIN-MORALES, M. A.. Toxic effects of Arianor Ebony hair dye on human cells. Brazilian Journal of Medical and Biological Research, v. 56, p. 9-pg., 2023-01-01. (13/21186-5, 10/11820-0)