Transcriptome Profile of Trichoderma harzianum IOC-3844 Induced by Sugarcane Bagasse

• Grant number: 14/01786-0
• Support Opportunities: Regular Research Grants - Publications - Scientific article
• Start date: March 01, 2014
• End date: August 31, 2014
• Field of knowledge: Agronomical Sciences - Agronomy
• Principal Investigator: Anete Pereira de Souza
• Grantee: Anete Pereira de Souza
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Profiling the transcriptome that underlies biomass degradation by the fungus Trichoderma harzianum allows for the identification of gene sequences with potential application in enzymatic hydrolysis processing. In the present study, the transcriptome of T. harzianum IOC-3844 was analyzed using RNA-seq technology. The sequencing generated 14.7 Gbp for downstream analyses. De novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities. This analysis allowed us to define a principal set of T. harzianum genes that are involved in the degradation of cellulose and hemicellulose and the accessory genes that are involved in the depolymerization of biomass. An additional analysis of expression levels identified a set of carbohydrate-active enzymes that are upregulated under different conditions. The present study provides valuable information for future studies on biomass degradation and contributes to a better understanding of the role of the genes that are involved in this process. (AU)