Mygalin: the immunomodulatory mechanism and potential in the control of bacterial infection

• Grant number: 13/11212-9
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2014
• End date: February 28, 2017
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Monamaris Marques Borges
• Grantee: Monamaris Marques Borges
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Marcia Regina Franzolin ; Maria Notomi Sato ; Maristela Martins de Camargo ; Pedro Ismael da Silva Junior

Abstract

The emergence of antibiotic-resistant pathogens emphasizes the need for alternative studies in fighting infections by identifying molecules that may act directly or indirectly on the immune response. The natural polyamines are present in all living cells binding to DNA and RNA regulating protein synthesis, cell differentiation, intracellular signaling and immune functions that may act or not as suppressive and anti-inflammatory depending on the number of amine groups present in the molecule, which gives its effector function. Mygalin is a synthetic analogue of the natural polyamine spermidine isolated from the spider Acanthoscurria gomesiana hemocytes. It induces the synthesis of IFN-g and NO in splenocytes cultures. The macrophages activation with Migalina increases NO and TNF-a but not IL-1². The addition of the compound to the splenocyte's culture did not stimulate IL-5 production and apoptosis of splenocytes and macrophages, nor did it affect splenocytes proliferation induced by ConA. It shows that the synthetic Mygalin modulates the innate immune response by activating phagocytes and probably the adaptative immunity during the infection control, fact that deserves further investigation. We will study the mechanism of action of Migalina (acylpolyamine) on the synthesis of inflammatory mediators during the innate response and its contribution to the control of infections by pathogenic bacteria. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIEIRA, PAULO C. G.; ESPINOZA-CULUPU, ABRAHAM O.; NEPOMUCENO, ROBERTO; ALVES, MARINA R.; LEBRUN, IVO; ELIAS, WALDIR P.; RUIZ, RITA C.. Secreted autotransporter toxin (Sat) induces cell damage during enteroaggregative Escherichia coli infection. PLoS One, v. 15, n. 2, FEB 21 2020. (13/11212-9)

ESPINOZA-CULUPU, ABRAHAM; VAZQUEZ-RAMIREZ, RICARDO; FARFAN-LOPEZ, MARIELLA; MENDES, ELIZABETH; NOTOMI SATO, MARIA; DA SILVA JUNIOR, PEDRO ISMAEL; BORGES, MONAMARIS MARQUES. Acylpolyamine Mygalin as a TLR4 Antagonist Based on Molecular Docking and In Vitro Analyses. BIOMOLECULES, v. 10, n. 12, DEC 2020. (13/11212-9, 13/07467-1)

ESPINOZA-CULUPU, ABRAHAM; MENDES, ELIZABETH; VITORINO, HECTOR AGUILAR; DA SILVA JR, PEDRO ISMAEL; BORGES, MONAMARIS MARQUES. Mygalin: An Acylpolyamine With Bactericidal Activity. FRONTIERS IN MICROBIOLOGY, v. 10, JAN 10 2020. (13/11212-9, 13/07467-1)