Effect of low-level laser therapy and growth factors on gingival fibroblasts activity of young and aged patients

• Grant number: 13/05879-0
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2014
• End date: January 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Carlos Alberto de Souza Costa
• Grantee: Carlos Alberto de Souza Costa
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated researchers: Fernanda Gonçalves Basso ; Josimeri Hebling Costa

Abstract

Tissue repair is related to several local events, such as cell proliferation and matrix protein synthesis, like collagen, elastin and reticular fibrils, which are directly associated to cell metabolism. Aging causes decrease of these cell functions, which can lead to the delay of wound repair. Gingival fibroblasts show greater repair capacity when compared to skin fibroblasts, but no studies have presented the influence of age on oral mucosa repair capacity as already shown for skin. Recent studies demonstrated that low-level laser therapy (LLLT) promotes biostimulation of different cell types and growth factors application, such as epithelial growth factors (EGF) accelerate repair process by increasing cell proliferation and migration capacity. Therefore, the present study aims to investigate cell metabolism, proliferation and migration capacity, which are cell functions related to repair capacity, of gingival fibroblasts of young and aged patients submitted or not to LLLT and treatment with growth factors. (AU)

Scientific publications

(18)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BASSO, FERNANDA G.; SOARES, DIANA G.; PANSANI, TAISA N.; CARDOSO, LAIS M.; SCHEFFEL, DEBORA L.; DE SOUZA COSTA, CARLOS ALBERTO; HEBLING, JOSIMERI. Proliferation, Migration, and Expression of Oral-Mucosal-Healing-Related Genes by Oral Fibroblasts Receiving Low-Level Laser Therapy After Inflammatory Cytokines Challenge. Lasers in Surgery and Medicine, v. 48, n. 10, SI, p. 1006-1014, DEC 2016. (13/05879-0, 12/17947-8)

SOARES, DIANA G.; BASSO, FERNANDA G.; SCHEFFEL, DEBORA SALLES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS A.. Responses of human dental pulp cells after application of a low-concentration bleaching gel to enamel. ARCHIVES OF ORAL BIOLOGY, v. 60, n. 9, p. 1428-1436, SEP 2015. (13/23520-0, 13/05879-0)

BASSO, F. G.; SOARES, D. G.; PANSANI, T. N.; TURRIONI, A. P. S.; SCHEFFEL, D. L.; DE SOUZA COSTA, C. A.; HEBLING, J.. Effect of LPS treatment on the viability and chemokine synthesis by epithelial cells and gingival fibroblasts. ARCHIVES OF ORAL BIOLOGY, v. 60, n. 8, p. 1117-1121, AUG 2015. (13/05879-0, 12/17947-8)

BASSO, F. G.; TURRIONI, A. P. S.; ALMEIDA, L. F.; SOARES, D. G.; OLIVEIRA, C. F.; HEBLING, J.; DE SOUZA COSTA, C. A.. Nutritional deprivation and LPS exposure as feasible methods for induction of cellular - A methodology to validate for vitro photobiomodulation studies. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 159, p. 205-210, JUN 2016. (13/05879-0, 12/17947-8)

BASSO, FERNANDA G.; SOARES, DIANA G.; DE SOUZA COSTA, CARLOS ALBERTO; HEBLING, JOSIMERI. Low-level laser therapy in 3D cell culture model using gingival fibroblasts. Lasers in Medical Science, v. 31, n. 5, p. 973-978, JUL 2016. (13/05879-0, 12/17947-8)

BASSO, FERNANDA G.; PANSANI, TAISA N.; TURRIONI, ANA PAULA S.; SOARES, DIANA G.; DE SOUZA COSTA, CARLOS ALBERTO; HEBLING, JOSIMERI. Tumor Necrosis Factor-alpha and Interleukin (IL)-1 beta, IL-6, and IL-8 Impair In Vitro Migration and Induce Apoptosis of Gingival Fibroblasts and Epithelial Cells, Delaying Wound Healing. Journal of Periodontology, v. 87, n. 8, p. 990-996, AUG 2016. (13/05879-0, 12/17947-8)

BASSO, FERNANDA G.; SOARES, DIANA G.; PANSANI, TAISA N.; CARDOSO, LAIS M.; SCHEFFEL, DEBORA L.; DE SOUZA COSTA, CARLOS ALBERTO; HEBLING, JOSIMERI. Proliferation, Migration, and Expression of Oral-Mucosal-Healing-Related Genes by Oral Fibroblasts Receiving Low-Level Laser Therapy After Inflammatory Cytokines Challenge. Lasers in Surgery and Medicine, v. 48, n. 10, p. 9-pg., 2016-12-01. (13/05879-0, 12/17947-8)

BASSO, FERNANDA GONCALVES; PANSANI, TAISA NOGUEIRA; CARDOSO, LAIS MEDEIROS; CITTA, MARIANA; SOARES, DIANA GABRIELA; SCHEFFEL, DEBORA SALLES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Epithelial cell-enhanced metabolism by low-level laser therapy and epidermal growth factor. Lasers in Medical Science, v. 33, n. 2, p. 445-449, FEB 2018. (13/05879-0)

BASSO, FERNANDA G.; PANSANI, TAISA N.; SOARES, DIANA G.; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. LLLT Effects on Oral Keratinocytes in an Organotypic 3D Model. Photochemistry and Photobiology, v. 94, n. 1, p. 190-194, JAN-FEB 2018. (14/06057-7, 13/05879-0, 12/17947-8)

PANSANI, TAISA NOGUEIRA; BASSO, FERNANDA GONCALVES; SILVEIRA TURRIONI, ANA PAULA; SOARES, DIANA GABRIELA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Effects of low-level laser therapy and epidermal growth factor on the activities of gingival fibroblasts obtained from young or elderly individuals. Lasers in Medical Science, v. 32, n. 1, p. 45-52, JAN 2017. (13/05879-0, 12/25070-9)

BASSO, FERNANDA GONCALVES; SOARES, DIANA GABRIELA; PANSANI, TAISA NOGUEIRA; SILVEIRA TURRIONI, ANA PAULA; SCHEFFEL, DEBORA LOPES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Response of a co-culture model of epithelial cells and gingival fibroblasts to zoledronic acid. Brazilian Oral Research, v. 30, n. 1, 2016. (13/05879-0, 12/17947-8)

BASSO, F. G.; HEBLING, J.; MARCELO, C. L.; COSTA, C. A. DE SOUZA; FEINBERG, S. E.. Development of an oral mucosa equivalent using a porcine dermal matrix. BRITISH JOURNAL OF ORAL & MAXILLOFACIAL SURGERY, v. 55, n. 3, p. 308-311, APR 2017. (12/17947-8, 14/06057-7, 13/05879-0)

SOARES, D. G.; BRITO, C. A.; TAVARES DA SILVA, R. H. B.; RIBEIRO, A. P. D.; HEBLING, J.; DE SOUZA COSTA, C. A.. Cytocompatibility of HEMA-free resin-based luting cements according to application protocols on dentine surfaces. International Endodontic Journal, v. 49, n. 6, p. 551-560, JUN 2016. (13/23520-0, 13/05879-0)

BASSO, FERNANDA G.; PANSANI, TAISA N.; MARCELO, CYNTIA L.; DE SOUZA COST, CARLOS ALBERTO; HEBLING, JOSIMERI; FEINBERG, STEPHEN E.. Phenotypic markers of oral keratinocytes seeded on two distinct 3D oral mucosa models. TOXICOLOGY IN VITRO, v. 51, p. 34-39, SEP 2018. (13/05879-0, 12/17947-8, 14/06057-7)

ROBERTI GARCIA, LUCAS DA FONSECA; VOLTOLINI PONTES, ELAINE CRISTINA; BASSO, FERNANDA GONCALVES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO; SOARES, DIANA GABRIELA. Transdentinal cytotoxicity of resin-based luting cements to pulp cells. CLINICAL ORAL INVESTIGATIONS, v. 20, n. 7, p. 1559-1566, SEP 2016. (13/23520-0, 13/05879-0, 12/14912-9)

BASSO, FERNANDA GONCALVES; SILVEIRA TURRIONI, ANA PAULA; SOARES, DIANA GABIELA; BAGNATO, VANDERLEI SALVADOR; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Low-level laser therapy for osteonecrotic lesions: effects on osteoblasts treated with zoledronic acid. SUPPORTIVE CARE IN CANCER, v. 22, n. 10, p. 2741-2748, OCT 2014. (13/05879-0, 12/17947-8)

BASSO, FERNANDA G.; PANSANI, TAISA N.; SOARES, DIANA G.; SCHEFFEL, DEBORA L.; BAGNATO, VANDERLEI S.; DE SOUZA COSTA, CARLOS ALBERTO; HEBLING, JOSIMERI. Biomodulation of Inflammatory Cytokines Related to Oral Mucositis by Low-Level Laser Therapy. Photochemistry and Photobiology, v. 91, n. 4, p. 952-956, JUL-AUG 2015. (13/05879-0, 12/17947-8, 14/06057-7)

BASSO, FERNANDA GONCALVES; SOARES, DIANA GABRIELA; PANSANI, TAISA NOGUEIRA; SILVEIRA TURRIONI, ANA PAULA; SCHEFFEL, DEBORA LOPES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Response of a co-culture model of epithelial cells and gingival fibroblasts to zoledronic acid. BRAZILIAN ORAL RESEARCH, v. 30, n. 1, p. 7-pg., 2016-01-01. (13/05879-0, 12/17947-8)