The role of the magnitude of muscle damage in hypertrophic responses

• Grant number: 13/21218-4
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2014
• End date: September 30, 2016
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Cleiton Augusto Libardi
• Grantee: Cleiton Augusto Libardi
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated researchers: Alexandre Giovinazzo Fochi ; Carlos Ugrinowitsch ; Felipe Romano Damas Nogueira ; Hamilton Augusto Roschel da Silva ; Manoel Emílio Lixandrão ; Patricia Chakur Brum ; Sanmy Rocha Nóbrega ; Valmor Alberto Augusto Tricoli

Abstract

It is not yet established whether muscle damage plays an important role in the chronic hypertrophic process in response to strength training in humans, since the magnitude of muscle damage is significantly attenuated throughout training sessions. Therefore, the aim of this study is investigate if the attenuation of muscle damage during training sessions reduces the rate of myofibrillar protein synthesis and activation of the intracellular mechanism of protein synthesis and, consequently, the magnitude of the hypertrophic response during 12 weeks of strength training in young men. It will be realized functional evaluations and muscle biopsies (before and immediately after the first, fifth and last training sessions) that will allow the analysis of the different magnitudes of muscle damage and their associated hypertrophic responses. The extent of damage will be assessed by quantification of maximum torque, sarcomeral Z-line integrity, disrupted desmin, ultrasound echo intensity. The rate of myofibrillar protein synthesis will be quantified through oral ingestion of deuterated water and muscle biopsies and the activation of hypertrophic mechanisms will be evaluated through the activation, proliferation and differentiation of satellite cells and myonuclei incorporation, and the responses of interleukin 15, fibroblast growth factor and growth factor induced by mechanical stress. The increase in muscle cross-sectional area will be evaluated both in whole muscle by the reconstruction technique of ultrasound images and in isolated fibers by histochemical analysis. Our hypothesis is that greater magnitudes of muscle damage (which will occur at the beginning of the training period) will induce a higher rate of myofibrillar protein synthesis and activation of the hypertrophic mechanism and with the course of training, the magnitude of damage will progressively be attenuated and the hypertrophic responses will also gradually decrease. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DAMAS, FELIPE; BARCELOS, CINTIA; NOBREGA, SANMY R.; UGRINOWITSCH, CARLOS; LIXANDRAO, MANOEL E.; SANTOS, LUCAS M. E. D.; CONCEICAO, MIGUEL S.; VECHIN, FELIPE C.; LIBARDI, CLEITON A.. INDIVIDUAL MUSCLE HYPERTROPHY AND STRENGTH RESPONSES TO HIGH VS. LOW RESISTANCE TRAINING FREQUENCIES. JOURNAL OF STRENGTH AND CONDITIONING RESEARCH, v. 33, n. 4, p. 897-901, APR 2019. (17/04299-1, 16/22635-6, 16/24259-1, 13/21218-4)

DAMAS, FELIPE; PHILLIPS, STUART M.; LIBARDI, CLEITON A.; VECHIN, FELIPE C.; LIXANDRAO, MANOEL E.; JANNIG, PAULO R.; COSTA, LUIZ A. R.; BACURAU, ALINE V.; SNIJDERS, TIM; PARISE, GIANNI; et al. Resistance training-induced changes in integrated myofibrillar protein synthesis are related to hypertrophy only after attenuation of muscle damage. JOURNAL OF PHYSIOLOGY-LONDON, v. 594, n. 18, p. 5209-5222, SEP 15 2016. (14/19594-0, 12/24499-1, 13/21218-4)

DAMAS, F.; NOSAKA, K.; LIBARDI, C. A.; CHEN, T. C.; UGRINOWITSCH, C.. Susceptibility to Exercise-Induced Muscle Damage: a Cluster Analysis with a Large Sample. INTERNATIONAL JOURNAL OF SPORTS MEDICINE, v. 37, n. 8, p. 633-640, JUL 2016. (14/19594-0, 12/24499-1, 13/21218-4)

FOCHI, A. G.; DAMAS, F.; BERTON, R.; ALVAREZ, I; MIQUELINI, M.; SALVINI, T. F.; LIBARDI, C. A.. Greater eccentric exercise-induced muscle damage by large versus small range of motion with the same end-point. BIOLOGY OF SPORT, v. 33, n. 3, p. 285-289, 2016. (12/24499-1, 11/22122-5, 14/19594-0, 13/21218-4)

NOBREGA, SANMY R.; UGRINOWITSCH, CARLOS; PINTANEL, LUCAS; BARCELOS, CINTIA; LIBARDI, CLEITON A.. EFFECT OF RESISTANCE TRAINING TO MUSCLE FAILURE VS. VOLITIONAL INTERRUPTION AT HIGH- AND LOW-INTENSITIES ON MUSCLE MASS AND STRENGTH. JOURNAL OF STRENGTH AND CONDITIONING RESEARCH, v. 32, n. 1, p. 162-169, JAN 2018. (13/21218-4, 15/05650-9)

DAMAS, FELIPE; UGRINOWITSCH, CARLOS; LIBARDI, CLEITON A.; JANNIG, PAULO R.; HECTOR, AMY J.; MCGLORY, CHRIS; LIXANDRAO, MANOEL E.; VECHIN, FELIPE C.; MONTENEGRO, HORACIO; TRICOLI, VALMOR; et al. Resistance training in young men induces muscle transcriptome-wide changes associated with muscle structure and metabolism refining the response to exercise-induced stress. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, v. 118, n. 12, p. 2607-2616, DEC 2018. (14/19594-0, 16/24259-1, 17/04299-1, 12/24499-1, 13/21218-4, 18/13064-0)

DAMAS, F.; LIBARDI, C. A.; UGRINOWITSCH, C.; VECHIN, F. C.; LIXANDRAO, M. E.; SNIJDERS, T.. Early- and later-phases satellite cell responses and myonuclear content with resistance training in young men (vol 13, e0191039, 2018). PLoS One, v. 13, n. 2, p. 1-pg., 2018-02-14. (16/24259-1, 13/21218-4, 12/24499-1, 14/19594-0)

DAMAS, FELIPE; LIBARDI, CLEITON A.; UGRINOWITSCH, CARLOS; VECHIN, FELIPE C.; LIXANDRAO, MANOEL E.; SNIJDERS, TIM; NEDERVEEN, JOSHUA P.; BACURAU, ALINE V.; BRUM, PATRICIA; TRICOLI, VALMOR; et al. Early- and later-phases satellite cell responses and myonuclear content with resistance training in young men. PLoS One, v. 13, n. 1, JAN 11 2018. (14/19594-0, 16/24259-1, 12/24499-1, 13/21218-4)

ANGLERI, VITOR; UGRINOWITSCH, CARLOS; LIBARDI, CLEITON AUGUSTO. Crescent pyramid and drop-set systems do not promote greater strength gains, muscle hypertrophy, and changes on muscle architecture compared with traditional resistance training in well-trained men. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, v. 117, n. 2, p. 359-369, FEB 2017. (15/16090-4, 13/21218-4)

DAMAS, FELIPE; PHILLIPS, STUART M.; LIXANDRAO, MANOEL E.; VECHIN, FELIPE C.; LIBARDI, CLEITON A.; ROSCHEL, HAMILTON; TRICOLI, VALMOR; UGRINOWITSCH, CARLOS. Early resistance training-induced increases in muscle cross-sectional area are concomitant with edema-induced muscle swelling. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, v. 116, n. 1, p. 49-56, JAN 2016. (14/19594-0, 12/24499-1, 13/21218-4)

DAMAS, FELIPE; PHILLIPS, STUART M.; LIBARDI, CLEITON A.; VECHIN, FELIPE C.; LIXANDRAO, MANOEL E.; JANNIG, PAULO R.; ROSCHEL, HAMILTON; COSTA, LUIZ A. R.; TRICOLI, VALMOR; UGRINOWITSCH, CARLOS. Muscle Damage Over A Resistance-training Period: Repeated Bout Effect Analysed Through Direct And Indirect Markers. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 48, n. 5, p. 1-pg., 2016-05-01. (12/24499-1, 13/21218-4, 14/19594-0)

ALVAREZ, IEDA FERNANDA; DAMAS, FELIPE; PIRES DE BIAZON, THAIS MARINA; MIQUELINI, MAIARA; DOMA, KENJI; LIBARDI, CLEITON AUGUSTO. Muscle damage responses to resistance exercise performed with high-load versus low-load associated with partial blood flow restriction in young women. European Journal of Sport Science, v. 20, n. 1, MAY 2019. (16/24259-1, 13/21218-4, 18/13064-0)

DAMAS, FELIPE; LIBARDI, CLEITON A.; UGRINOWITSCH, CARLOS. The development of skeletal muscle hypertrophy through resistance training: the role of muscle damage and muscle protein synthesis. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, v. 118, n. 3, p. 485-500, MAR 2018. (14/19594-0, 16/24259-1, 17/04299-1, 12/24499-1, 13/21218-4)